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Self-assembling protein–polymer bioconjugates for surfaces with antifouling features and low nonspecific binding
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Liu, Yingying, Nevanen, Tarja K., Paananen, Arja, Kempe, Kristian, Wilson, Paul, Johansson, Leena-Sisko, Joensuu, Jussi J., Linder, Markus B., Haddleton, David M. and Milani, Roberto (2019) Self-assembling protein–polymer bioconjugates for surfaces with antifouling features and low nonspecific binding. ACS Applied Materials & Interfaces, 11 (3). pp. 3599-3608. doi:10.1021/acsami.8b19968 ISSN 1944-8244.
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Official URL: http://dx.doi.org/10.1021/acsami.8b19968
Abstract
A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Self-assembly (Chemistry), Proteins, Polymers, Bioconjugates, Hydrophobic surfaces | |||||||||||||||
Journal or Publication Title: | ACS Applied Materials & Interfaces | |||||||||||||||
Publisher: | American Chemical Society | |||||||||||||||
ISSN: | 1944-8244 | |||||||||||||||
Official Date: | 23 January 2019 | |||||||||||||||
Dates: |
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Volume: | 11 | |||||||||||||||
Number: | 3 | |||||||||||||||
Page Range: | pp. 3599-3608 | |||||||||||||||
DOI: | 10.1021/acsami.8b19968 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 11 January 2019 | |||||||||||||||
Date of first compliant Open Access: | 16 January 2019 | |||||||||||||||
RIOXX Funder/Project Grant: |
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