Cevik, Volkan, Boutrot, Freddy, Apel, Wiebke, Robert-Seilaniantz, Alexandre, Furzer, Oliver J., Redkar, Amey, Castel, Baptiste, Kover, Paula, Prince, David C., Holub, E. B. and Jones, Jonathan D. G. (2019) Transgressive segregation reveals mechanisms of Arabidopsis immunity to Brassica-infecting races of white rust (Albugo candida). Proceedings of the National Academy of Sciences, 116 (7). pp. 2767-2773. doi:10.1073/pnas.1812911116 ISSN 0027-8424.

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Abstract

Arabidopsis thaliana accessions are universally resistant at the adult leaf stage to white rust (Albugo candida) races that infect the crop species Brassica juncea and Brassica oleracea. We used transgressive segregation in recombinant inbred lines to test if this apparent species-wide (nonhost) resistance in A. thaliana is due to natural pyramiding of multiple Resistance (R) genes. We screened 593 inbred lines from an Arabidopsis multiparent advanced generation intercross (MAGIC) mapping population, derived from 19 resistant parental accessions, and identified two transgressive segregants that are susceptible to the pathogen. These were crossed to each MAGIC parent, and analysis of resulting F2 progeny followed by positional cloning showed that resistance to an isolate of A. candida race 2 (Ac2V) can be explained in each accession by at least one of four genes encoding nucleotide-binding, leucine-rich repeat (NLR) immune receptors. An additional gene was identified that confers resistance to an isolate of A. candida race 9 (AcBoT) that infects B. oleracea. Thus, effector-triggered immunity conferred by distinct NLR-encoding genes in multiple A. thaliana accessions provides species-wide resistance to these crop pathogens.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Arabidopsis thaliana, Arabidopsis thaliana -- Diseases and pests, Oomycetes, Albugo candida, Albugo candida -- Detection, Natural immunity, Cruciferae, Cruciferae -- Diseases and pests
Journal or Publication Title:	Proceedings of the National Academy of Sciences
Publisher:	National Academy of Sciences
ISSN:	0027-8424
Official Date:	February 2019
Dates:	Date Event February 2019 Published 12 February 2019 Available 19 December 2018 Accepted
Volume:	116
Number:	7
Page Range:	pp. 2767-2773
DOI:	10.1073/pnas.1812911116
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Date of first compliant deposit:	15 January 2019
Date of first compliant Open Access:	19 February 2019
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID GBMF4725 Gordon and Betty Moore Foundation http://dx.doi.org/10.13039/100000936 Advanced InvestigatorGrant 233376 (ALBUGON) European Research Counci http://id.loc.gov/authorities/names/n90629676 B/L011646/1 [BBSRC] Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BB/M003809/1 [BBSRC] Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 Long-Term Fellowship ALTF-842-2015 European Molecular Biology Organization http://dx.doi.org/10.13039/100004410

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