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Editing streptomycete genomes in the CRISPR/Cas9 age
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Alberti, Fabrizio and Corre, Christophe (2019) Editing streptomycete genomes in the CRISPR/Cas9 age. Natural Product Reports, 36 (9). pp. 1237-1248. doi:10.1039/c8np00081f ISSN 0265-0568.
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Official URL: https://doi.org/10.1039/c8np00081f
Abstract
This article aims to highlight advantages, drawbacks and issues that users should consider when implementing the use of CRISPR/Cas9-tools for genome editing in streptomycetes, the most prolific source of antimicrobial natural products to date. Here, we examine four toolkits that have so far been made available for streptomycete in vivo-engineering and one for in vitro-editing, and review how they have been applied over the last three years. Our critical evaluation of these toolkits intends to support potential users in determining what they could achieve, what they should consider and what system they should select/optimise for their application.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QH Natural history | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Library of Congress Subject Headings (LCSH): | Gene editing, Genetic engineering, Anti-infective agents | ||||||||||||
| Journal or Publication Title: | Natural Product Reports | ||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||
| ISSN: | 0265-0568 | ||||||||||||
| Official Date: | 25 January 2019 | ||||||||||||
| Dates: |
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| Volume: | 36 | ||||||||||||
| Number: | 9 | ||||||||||||
| Page Range: | pp. 1237-1248 | ||||||||||||
| DOI: | 10.1039/c8np00081f | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
| Date of first compliant deposit: | 6 March 2019 | ||||||||||||
| Date of first compliant Open Access: | 5 April 2019 | ||||||||||||
| RIOXX Funder/Project Grant: |
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