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The antigenicity of the chicken anaemia virus protein VP3 (Apoptin)
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UNSPECIFIED (2001) The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). AVIAN PATHOLOGY, 30 (6). pp. 613-619. ISSN 0307-9457.
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Abstract
Chicken anaemia virus protein VP3 (Apoptin) was cloned and expressed as a recombinant protein and evaluated for its suitability as a serodiagnostic reagent. VP3 was expressed as a fusion protein either with glutathione S-transferase or with a six-histidine tag. Both recombinant proteins reacted specifically with anti-VP3 monoclonal antibodies and with serum from vaccinated chickens by Western blot and by enzyme-linked immunosorbent assay (ELISA). However, when testing sera from birds of different ages and genetic backgrounds, high non-specific reactions were evident and false positives were observed, especially in older birds. This suggests that VP3 is poorly immunogenic during infection and low antibody concentrations are masked by non-specific reactions. Thus, VP3 is not suitable for use as antigen in ELISAs.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SF Animal culture | ||||
Journal or Publication Title: | AVIAN PATHOLOGY | ||||
Publisher: | CARFAX PUBLISHING | ||||
ISSN: | 0307-9457 | ||||
Official Date: | December 2001 | ||||
Dates: |
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Volume: | 30 | ||||
Number: | 6 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 613-619 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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