The Library
Expanding the zebrafish genetic code through site-specific introduction of azido-lysine, bicyclononyne-lysine, and diazirine-lysine
Tools
Tools
Tools
Syed, Junetha, Palani, Saravanan, Clarke, Scott T., Asad, Zainab, Bottrill, Andrew R., Jones, Alexandra M.E., Sampath, Karuna and Balasubramanian, Mohan K. (2019) Expanding the zebrafish genetic code through site-specific introduction of azido-lysine, bicyclononyne-lysine, and diazirine-lysine. International Journal of Molecular Sciences, 20 (10). 2577. doi:10.3390/ijms20102577 ISSN 1422-0067.
|
PDF
WRAP-expanding-zebrafish-genetic-code-Jones-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2620Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/ijms20102577
Abstract
Site-specific incorporation of un-natural amino acids (UNAA) is a powerful approach to engineer and understand protein function. Site-specific incorporation of UNAAs is achieved through repurposing the amber codon (UAG) as a sense codon for the UNAA, using a tRNACUA that base pairs with an UAG codon in the mRNA and an orthogonal amino-acyl tRNA synthetase (aaRS) that charges the tRNACUA with the UNAA. Here, we report an expansion of the zebrafish genetic code to incorporate the UNAAs, azido-lysine (AzK), bicyclononyne-lysine (BCNK), and diazirine-lysine (AbK) into green fluorescent protein (GFP) and glutathione-s-transferase (GST). We also present proteomic evidence for UNAA incorporation into GFP. Our work sets the stage for the use of AzK, BCNK, and AbK introduction into proteins as a means to investigate and engineer their function in zebrafish.
| Item Type: | Journal Article | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||
| Journal or Publication Title: | International Journal of Molecular Sciences | |||||||||||||||
| Publisher: | M D P I AG | |||||||||||||||
| ISSN: | 1422-0067 | |||||||||||||||
| Official Date: | 26 May 2019 | |||||||||||||||
| Dates: |
|
|||||||||||||||
| Volume: | 20 | |||||||||||||||
| Number: | 10 | |||||||||||||||
| Article Number: | 2577 | |||||||||||||||
| DOI: | 10.3390/ijms20102577 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 26 June 2019 | |||||||||||||||
| Date of first compliant Open Access: | 26 June 2019 | |||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
