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Analysis of intact bacteriohopanepolyols from methanotrophic bacteria by reversed-phase high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry
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UNSPECIFIED (2001) Analysis of intact bacteriohopanepolyols from methanotrophic bacteria by reversed-phase high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 921 (2). pp. 175-185. ISSN 0021-9673.
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Abstract
Direct detection of most intact biohopanoids is not possible using conventional GC-MS techniques due to their highly functionalised and amphiphilic nature. Here we report the application of a new reversed-phase high-performance liquid chromatography method for the direct analysis of acetylated, intact bacteriohopanepolyols in solvent extracts of methanotrophic bacteria. Atmospheric pressure chemical ionisation mass spectrometric detection provides structural information relating to the number and types of functional groups present in the four biohopanoids detected: bacteriohopanetetrol, aminobacteriohopanetriol, -tetrol and -pentol. The method should facilitate the assessment of hopanoid composition of both bacteria and environmental samples. (C) 2001 Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | JOURNAL OF CHROMATOGRAPHY A | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0021-9673 | ||||
Official Date: | 6 July 2001 | ||||
Dates: |
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Volume: | 921 | ||||
Number: | 2 | ||||
Number of Pages: | 11 | ||||
Page Range: | pp. 175-185 | ||||
Publication Status: | Published |
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