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A suite of solid-state NMR experiments to utilize orphaned magnetization for assigning proteins using parallel high and low gamma detection
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Gallo, Angelo, Franks, W. Trent and Lewandowski, Józef R. (2019) A suite of solid-state NMR experiments to utilize orphaned magnetization for assigning proteins using parallel high and low gamma detection. Journal of Magnetic Resonance, 305 . pp. 219-231. doi:10.1016/j.jmr.2019.07.006 ISSN 1090-7807.
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Official URL: https://doi.org/10.1016/j.jmr.2019.07.006
Abstract
We present a suite of two-receiver solid-state NMR experiments for backbone and side chain resonance assignment. The experiments rely on either dipolar coupling or scalar coupling for polarization transfer and are devised to acquire a 1H–detected 3D experiment AND a nested 13C–detected 2D from a shared excitation pulse. In order to compensate for the lower sensitivity of detection on 13C nucleus, 2D rows are signal averaged during 3D planes. The 3D dual receiver experiments do not suffer from any appreciable signal loss compared to their single receiver versions and require no extra optimization. The resulting data is higher in information content with no additional experiment time. The approach is expected to become widespread as multiple receivers become standard for new NMR spectrometers.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics |
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Library of Congress Subject Headings (LCSH): | Nuclear magnetic resonance spectroscopy, Proteins | ||||||||||||
Journal or Publication Title: | Journal of Magnetic Resonance | ||||||||||||
Publisher: | Academic Press | ||||||||||||
ISSN: | 1090-7807 | ||||||||||||
Official Date: | August 2019 | ||||||||||||
Dates: |
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Volume: | 305 | ||||||||||||
Page Range: | pp. 219-231 | ||||||||||||
DOI: | 10.1016/j.jmr.2019.07.006 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 26 July 2019 | ||||||||||||
Date of first compliant Open Access: | 29 July 2019 | ||||||||||||
RIOXX Funder/Project Grant: |
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