The Library
Structure of the maize streak virus geminate particle
Tools
UNSPECIFIED (2001) Structure of the maize streak virus geminate particle. VIROLOGY, 279 (2). pp. 471-477. doi:10.1006/viro.2000.0739 ISSN 0042-6822.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1006/viro.2000.0739
Abstract
lThe Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstruction methods. The particle, of dimensions 220 x 380 Angstrom, has an overall 52-point-group symmetry, in which each half particle "head" consists of the coal protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel beta -barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal alpha -helix. This has produced a model of the geminate particle in which 110 copies of the CP nicely fit into the reconstructed density map. The reconstructed density map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure. (C) 2001 Academic Press.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||
Journal or Publication Title: | VIROLOGY | ||||
Publisher: | ACADEMIC PRESS INC | ||||
ISSN: | 0042-6822 | ||||
Official Date: | 20 January 2001 | ||||
Dates: |
|
||||
Volume: | 279 | ||||
Number: | 2 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 471-477 | ||||
DOI: | 10.1006/viro.2000.0739 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |