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Control of developmental reprogramming during somatic cell regeneration in Arabidopsis thaliana
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Naish, Matthew (2018) Control of developmental reprogramming during somatic cell regeneration in Arabidopsis thaliana. PhD thesis, University of Warwick.
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WRAP_Theses_Naish_2019.pdf - Submitted Version - Requires a PDF viewer. Download (35Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3422942~S15
Abstract
Unlike most animals, plants can regenerate new tissues, organs or an entire organism by changing the developmentally committed program of individual cells - a phenomenon known as totipotency. This unique developmental plasticity of plants has been exploited in agriculture and horticulture to generate clonal plants in axenic culture through the induction of a somatic embryogenesis. However, some plant species are resistant to current regeneration protocols and the precise mechanisms responsible for totipotency in plant cells remain unknown. Using a novel system to induce somatic embryos from terminally differentiated somatic cells, we have investigated the importance of histone modifying proteins belonging to the Polycomb group (PcG) and Jumonji C-domain-containing class (JmJ-C), on cellular susceptibility to developmental reprogramming. We found that reprogramming success is influenced by cell type, developmental stage and that JmJ-C proteins play an important role in facilitating plant cellular plasticity. We also identified a protective role against epimutations during sexual reproduction in Arabidopsis thaliana. Furthermore, this system enabled the identification of key factors involved in the transition away from a differentiated state and into a new developmental fate, providing insights into the regulatory mechanism of totipotency in plants.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
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Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana, Somatic cells -- Regeneration, Somatic embryogenesis, Plants -- Reproduction | ||||
Official Date: | September 2018 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Gutierrez-Marcos, José F. | ||||
Sponsors: | Midlands Integrative Biosciences Training Partnership | ||||
Format of File: | |||||
Extent: | xiii, 162 leaves : illustrations, charts | ||||
Language: | eng |
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