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Regulation of expression of the glutamine synthetase GLN-[alpha] gene of phaseolus vulgaris L
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Daniell, Timothy John (1992) Regulation of expression of the glutamine synthetase GLN-[alpha] gene of phaseolus vulgaris L. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b1415234~S15
Abstract
The expression of the glutamine synthetase a subunit gene (gln-a) is known to be high in young tissues such as root tips, plumules developing nodules and developing leaves. An experiment using nodules were Fix- and began to senesce prematurely, suggested that gln-a and a phenylalanine ammonia-lyase gene (PAL 1) were co-induced.
This phenomenon was further studied in various systems including nodules derived with Rhizobium mutants, cell cultures treated with fungal elicitors, wounded hypocotyls and leaves infected with compatible and incompatible strains of Pseudomonas phaseolicola. Expression of these two genes and in some cases the glutamine synthetase ß subunit gene (gln-ß) was observed to be related although experiments with short periods between samples suggested that the expression of the two glutamine synthetase genes was delayed with respect to the expression of the PAL 1 gene, the peak of expression occuring a few hours later. Work on cell cultures suggests that the stimulus for the induction of the two glutamine synthetase genes is possibly a product of the phenylpropanoid pathway although other stimuli present in both stress conditions and in young tissues may induce either of the two glutamine synthetase genes
| Item Type: | Thesis or Dissertation (PhD) | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany | ||||
| Library of Congress Subject Headings (LCSH): | Plant genetics, Glutamine synthetase, Roots (Botany), Plant gene expression | ||||
| Official Date: | September 1992 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | Department of Biological Sciences | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Cullimore, Julie | ||||
| Sponsors: | Science and Engineering Research Council (Great Britain) | ||||
| Extent: | 177 leaves : illustrations | ||||
| Language: | eng |
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