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An RNAi suppressor activates in planta virus-mediated gene editing
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Zhang, Xian, Kang, Lihua, Zhang, Qi, Meng, Qiqi, Pan, Yafei, Yu, Zhiming, Shi, Nongnong, Jackson, Stephen D. , Zhang, Xinlian, Wang, Huizhong, Tör, Mahmut and Hong, Yiguo (2020) An RNAi suppressor activates in planta virus-mediated gene editing. Functional & Integrative Genomics, 20 . pp. 471-477. doi:10.1007/s10142-019-00730-y ISSN 1438-793X.
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WRAP-RNAi-suppressor-activates-virus-gene-editing-Hong-2020.pdf - Accepted Version - Requires a PDF viewer. Download (1174Kb) | Preview |
Official URL: https://doi.org/10.1007/s10142-019-00730-y
Abstract
RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Antisense RNA, Catalytic RNA, Gene editing , CRISPR-associated protein 9 | ||||||||||||||||||||||||
Journal or Publication Title: | Functional & Integrative Genomics | ||||||||||||||||||||||||
Publisher: | Springer | ||||||||||||||||||||||||
ISSN: | 1438-793X | ||||||||||||||||||||||||
Official Date: | July 2020 | ||||||||||||||||||||||||
Dates: |
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Volume: | 20 | ||||||||||||||||||||||||
Page Range: | pp. 471-477 | ||||||||||||||||||||||||
DOI: | 10.1007/s10142-019-00730-y | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | This is a post-peer-review, pre-copyedit version of an article published in Functional & Integrative Genomics. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10142-019-00730-y | ||||||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||||||||
Date of first compliant deposit: | 12 February 2020 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 19 December 2020 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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