The Library
IGS region polymorphisms are responsible for failure of commonly used species‐specific primers in Fusarium proliferatum isolates from diseased garlic
Tools
Gálvez, L., Clarkson, John P. and Palmero, D. (2020) IGS region polymorphisms are responsible for failure of commonly used species‐specific primers in Fusarium proliferatum isolates from diseased garlic. Plant Pathology, 69 (4). pp. 713-722. doi:10.1111/ppa.13162 ISSN 1365-3059.
|
PDF
WRAP-IGS-region-polymorphisms-failure-proliferatum-isolates-garlic-Clarkson-2020.pdf - Accepted Version - Requires a PDF viewer. Download (2045Kb) | Preview |
Official URL: https://doi.org/10.1111/ppa.13162
Abstract
Fusarium proliferatum is a globally distributed fungal pathogen that affects a range of crop hosts and is one of the main producers of mycotoxins, such as fumonisins, in foods. Specific PCR primers are commonly used for detection and identification of this pathogen. The aim of this study was to validate previously published F. proliferatum‐specific primers targeting the intergenic spacer (IGS) region and characterize intraspecific variation and homologous recombination events for isolates obtained from diseased garlic bulbs in Spain. Sixty‐nine isolates were morphologically identified as F. proliferatum, and their identity was confirmed by sequencing of the translation elongation factor; however, specific IGS primers did not result in an amplification product for nine of these isolates. Further analysis showed that this was due to polymorphism in the IGS region and six isolates were classified as IGS type I, while the remaining isolates were type II. Sequencing of the complete IGS region revealed numerous sequence polymorphisms amongst F. proliferatum isolates, and regions of recombination. Duplication and deletion events may have occurred via unequal crossing over during mitotic or meiotic recombination. These results suggest that the IGS region may be too variable as a reliable target for F. proliferatum‐specific identification.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | S Agriculture > SB Plant culture | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Library of Congress Subject Headings (LCSH): | Fusarium diseases of plants, Garlic -- Diseases and pest, Isoenzymes -- Pathophysiology | ||||||||
Journal or Publication Title: | Plant Pathology | ||||||||
Publisher: | Wiley | ||||||||
ISSN: | 1365-3059 | ||||||||
Official Date: | May 2020 | ||||||||
Dates: |
|
||||||||
Volume: | 69 | ||||||||
Number: | 4 | ||||||||
Page Range: | pp. 713-722 | ||||||||
DOI: | 10.1111/ppa.13162 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Reuse Statement (publisher, data, author rights): | "This is the peer reviewed version of the following article:Gálvez, L, Clarkson, JP, Palmero, D. IGS region polymorphisms are responsible for failure of commonly used species‐specific primers in Fusarium proliferatum isolates from diseased garlic. Plant Pathol. 2020; 00: 1– 10., which has been published in final form at https://doi.org/10.1111/ppa.13162. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 6 March 2020 | ||||||||
Date of first compliant Open Access: | 11 February 2021 | ||||||||
RIOXX Funder/Project Grant: |
|
||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year