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Myosin II filament dynamics in actin networks revealed with interferometric scattering microscopy
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Mosby, Lewis S., Hundt, N., Young, G., Fineberg, A., Polin, Marco, Mayor, S., Kukura, Philipp and Köster, Darius (2020) Myosin II filament dynamics in actin networks revealed with interferometric scattering microscopy. Biophysical Journal . doi:10.1016/j.bpj.2020.02.025 ISSN 0006-3495.
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WRAP-myosin-filament-dynamics-actin-networks-interferometric-microscopy-Koster-2020.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2966Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.bpj.2020.02.025
Abstract
The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood due to difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering (iSCAT) microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect, and image individual membrane attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify myosin II filament dwell times and processivity as functions of ATP concentration, providing experimental evidence for the predicted ensemble behavior of myosin head domains. Our results show how decreasing ATP concentrations lead to both increasing dwell times of individual myosin II filaments and a global change from a remodeling to a contractile state of the acto-myosin network.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QC Physics Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Interferometry, Scattering (Physics), Actomyosin, Microscopy, Microfilament proteins | ||||||||||||||||||||||||
Journal or Publication Title: | Biophysical Journal | ||||||||||||||||||||||||
Publisher: | Biophysical Society | ||||||||||||||||||||||||
ISSN: | 0006-3495 | ||||||||||||||||||||||||
Official Date: | 2020 | ||||||||||||||||||||||||
Dates: |
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DOI: | 10.1016/j.bpj.2020.02.025 | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Date of first compliant deposit: | 11 March 2020 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 18 March 2020 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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