Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Studies on the peripheral light-harvesting chlorophyll-protein complex of photosystem I in "Pisum sativum L."

Tools
- Tools
+ Tools

Williams, Richard Stephen (1987) Studies on the peripheral light-harvesting chlorophyll-protein complex of photosystem I in "Pisum sativum L.". PhD thesis, University of Warwick.

[img]
Preview
PDF
WRAP_Theses_Williams_1987.pdf - Unspecified Version - Requires a PDF viewer.

Download (6Mb) | Preview
Official URL: http://webcat.warwick.ac.uk/record=b1448684~S15

Request Changes to record.

Abstract

Two novel sub-thylakoid fractions from Pisum sativum L. containing the photosystem I reaction centre and peripheral light-harvesting complex were characterised. Both preparations displayed a number of spectral and biochemical properties in common with those exhibited by a standard photosystem I preparation. However, both differed markedly in their sedimentation properties when compared with the standard preparation.

In addition, one of the novel preparations was found to contain large amounts of the chlorophyll a/b-binding light-harvesting complex of photosystem II (LHC II). It was shown that excitation energy is transferred from the LHC II component to the photosystem I component within this preparation. Such excitation energy transfer has not previously been demonstrated in a sub- thylakoid preparation.

Monoclonal and polyclonal antibodies were raised to the apoproteins of the chlorophyll a/b-binding peripheral light-harvesting complex of photosystem I (LHC I). Assays of polyclonal antibody specificity showed immunological cross-reaction between individual LHC I apoproteins and between the apoproteins of the LHC I and LHC II.

The immunological cross-reactivity between the polypeptides of the LHC I and LHC II were shown to be spurious. The LHC I apoproteins were found to fall into two distinct immunological groupings which were supported by assays of monoclonal antibody specificity and by a comparison of their partial peptide maps.

Immunoblot assays showed that the LHC I apoproteins failed to accumulate in the thylakoid membrane under intern intermittent illumination. When greening pea seedlings were placed in the dark, the LHC I apoproteins not only failed to accumulate but underwent a net loss during the dark period. In vivo radiolabelling experiments showed that the apoproteins of the LHC I continued to be synthesised even after 16 h darkness. The failure of these proteins to accumulate in the thylakoid in the dark is therefore due to protein turnover.

Item Type: Thesis (PhD)
Subjects: Q Science > QD Chemistry
Q Science > QK Botany
Library of Congress Subject Headings (LCSH): Peas -- Analysis, Chlorophyll -- Synthesis, Proteins -- Analysis
Official Date: March 1987
Dates:
DateEvent
March 1987UNSPECIFIED
Institution: University of Warwick
Theses Department: Department of Biological Sciences
Thesis Type: PhD
Publication Status: Unpublished
Supervisor(s)/Advisor: Ellis, R. J. (Reginald John)
Sponsors: Science and Engineering Research Council (Great Britain)
Extent: xxi, 394 leaves : illustrations
Language: eng

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us