Rigg, Richard James (1987) Neutralisation of influenza virus : evidence that neutralisation by IgG is the result of incomplete uncoating rather than a reduction in the virion transcriptase activity. PhD thesis, University of Warwick.

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Abstract

This thesis is an account of experiments designed to investigate further the mechanism of neutralisation of influenza virus by immunoglobulin G (IgG) .

Section III demonstrates that neutralised virus does not direct primary transcription vivo thus extending the findings of Possee et al. (1982) who reported lack of detectable secondary transcription.

Evidence is presented in Section IV which suggests an explanation for the lack of transcription in vivo. Neutralised virus undergoes incomplete uncoating such that the mRNA does not become susceptible to RNase digestion. However, consistent with the findings of other workers, IgG-neutralised virus attached to cells and the mRNA became localised in the nucleus to the same extent as infectious virus. It is therefore proposed that neutralisation of influenza virus by IgG is the result of failure to uncoat sufficiently for the virion transcriptase enzyme to become active.

The transcriptase activity of neutralised influenza virus was also investigated in vitro using mRNA as primer. None of the cap-utilising functions were significantly inhibited (Section II). This suggests that not all neutralising antibodies cause the inhibition of transcription in vitro, first described by Possee et al. (1982). It is probable that disruption of the virus membrane in vitro does not accurately mimic the uncoating processes that take place in vitro.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QR Microbiology
Library of Congress Subject Headings (LCSH):	Influenza viruses -- Research, Neutralization (Chemistry), Immunoglobulin G
Official Date:	July 1987
Dates:	Date Event July 1987 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Dimmock, N. J.
Extent:	xix, 266 leaves : illustrations
Language:	eng

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