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Studies on bacterial gene transposition
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Wilkins, Gary Mark (1987) Studies on bacterial gene transposition. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b1452019~S15
Abstract
(1) Two Escherichia coli K12 based transposition frequency assay systems have been designed and developed to allow the frequency of prokaryotic transposable element movements to be simply assayed. One of these systems involves the giant transposon bacteriophage Mu dl(ApRlac), that carries the P-galactosidase gene so that insertion adjacent to promotors can be quantified. The other system developed involves transposon 5 (Tn5), initially inserted in the host genome, that encodes kanamycin resistance which allows transposition to (and amplification in) plasmids to be quantified.
(2) The most transposogenic compounds identified in the two transposition frequency assays are the alkyl-nitro-nitrosoguanidine alkylating agents. Most other alkylating agents examined were either weakly transposogenic or non-transposogenic.
(3) The alkyl-nitro-nitrosoguanidines appear to act by guanylate cyclase activation, via nitroxide radical release, and subsequent elevation of the cGMP concentration. The transposogen- icity of cGMP is confirmed by an apparant effect of permeable cGMP derivatives on Mu d\(ApRlac) and Tn5 transposition frequency.
(4) A group of metals were examined for transposogenicity, and showed that transposogeni- city may be related to mutagenicity since mutagenic metals (Cd(II), Cr(VI) and Mn(II)) are transposogenic and an anti-mutagenic metal (Co(II)) is anti-transposogenic.
(5) The majority of transposogens identified in the two transposition frequency assays were mutagens, well documented in literature, and the putative non-transposogens were non- mutagenic. However, one group of compounds investigated were three non-mutagenic (Ames test negative) carcinogens. Diethylstilbesuol, DDT and o-toluidine each gave increases in Mu d\{ApRlac) transposition frequency which, although weak effects, might not have been expected from the other data, which suggested that mutagenicity and transposogenicity are inter-connected
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||
Library of Congress Subject Headings (LCSH): | Transposons, Escherichia coli K12, Bacterial genetics -- Research, Gene expression | ||||
Official Date: | July 1987 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Department of Chemistry | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Swoboda, Bennett Edward Paul | ||||
Sponsors: | Science and Engineering Research Council (Great Britain), Imperial Chemical Industries, ltd., University of Warwick | ||||
Extent: | [21], 369 leaves | ||||
Language: | eng |
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