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Label-free nano-imaging of neuromelanin in the brain by soft x-ray spectromicroscopy
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Brooks, Jake, Everett, James, Lermyte, Frederik, Tjhin, Vindy Tjendana , Banerjee, Samya, O’Connor, Peter B., Morrison, Christopher M. , Sadler, Peter J., Telling, Neil D. and Collingwood, Joanna F. (2020) Label-free nano-imaging of neuromelanin in the brain by soft x-ray spectromicroscopy. Angewandte Chemie (International Edition), 59 (29). pp. 11984-11991. doi:10.1002/anie.202000239 ISSN 1433-7851.
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WRAP-label-free-nanoimaging-neuromelanin-brain-soft-x-ray-spectromicroscopy-Brooks-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1607Kb) | Preview |
Official URL: https://doi.org/10.1002/anie.202000239
Abstract
A hallmark of Parkinson's disease is the death of neuromelanin-pigmented neurons, but the role of neuromelanin is unclear. The in situ characterization of neuromelanin remains dependent on detectable pigmentation, rather than direct quantification of neuromelanin. We show that direct, label-free nanoscale visualization of neuromelanin and associated metal ions in human brain tissue can be achieved using synchrotron scanning transmission x-ray microscopy (STXM), through a characteristic feature in the neuromelanin x-ray absorption spectrum at 287.4 eV that is also present in iron-free and iron-laden synthetic neuromelanin. This is confirmed in consecutive brain sections by correlating STXM neuromelanin imaging with silver nitrate-stained neuromelanin. Analysis suggests that the 1s–σ* (C−S) transition in benzothiazine groups accounts for this feature. This method illustrates the wider potential of STXM as a label-free spectromicroscopy technique applicable to both organic and inorganic materials.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | R Medicine > R Medicine (General) R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Parkinson’s disease, Iron, Neuromelanin, X-ray spectroscopy, Nanomedicine, Nanostructured materials -- Optical properties | |||||||||||||||
Journal or Publication Title: | Angewandte Chemie (International Edition) | |||||||||||||||
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | |||||||||||||||
ISSN: | 1433-7851 | |||||||||||||||
Official Date: | July 2020 | |||||||||||||||
Dates: |
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Volume: | 59 | |||||||||||||||
Number: | 29 | |||||||||||||||
Page Range: | pp. 11984-11991 | |||||||||||||||
DOI: | 10.1002/anie.202000239 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 28 May 2021 | |||||||||||||||
Date of first compliant Open Access: | 28 May 2021 | |||||||||||||||
RIOXX Funder/Project Grant: |
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