Darlison, Mark Geoffrey (1979) Cytoplasmic mutants of yeast : the isolation and genetic characterization of cytoplasmic mutants of Saccharomyces cerevisiae defective in oxidative phosphorylation. PhD thesis, University of Warwick.

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Abstract

This thesis presents a method for the induction and selection of cytoplasmic respiratory deficient mutants of Saccharomyces cerevisiae, distinct from the pleiotropic rhoˉ petites. The procedure utilizes a parental stock carrying the nuclear recessive pet 9 (op₁) mutation. Mutagenesis is performed under growing conditions in the presence of manganese.

As a result of this treatment twenty one mutants, putatively designated mitˉ, have been isolated. Among these are strains that are defective in the biosynthesis of either cytochrome b or cytochrome oxidase.

Two mutants, D603-3B/8 and D603-3B/9, have been intensively studied following the observation that they possess spectrophot-metrically detectable levels of cytochromes c, b and a + a₃.
The mitˉ mutations carried by these strains have been further genetically characterized and mapped with respect to known mitochondrial markers. These lesions have been located close to, and are possibly allelic with, the pho 1 segment of the genome.

In addition, a very tight linkage to the oliʳ2 antibiotic resistance locus has been established.

Attempts to determine whether the mitˉ mutations of D603-3B/8 and D603-3E/9 are allelic, have proved unsuccessful.

The effect of these lesions, in constructed mitˉ diploids, on growth in glucose and glycerol liquid media has also been investigated.

It is suggested that D603-3B/8 and D603-3B/9 are defective in the biosynthesis of the CS-ATPase complex. Their potential use in studies of the biogenesis of this multisubunit enzyme is also discussed.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QH Natural history Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Saccharomyces cerevisiae, Yeast -- Molecular aspects, Cytoplasm, Plant mutation, Plants -- Phosphorylation
Official Date:	June 1979
Dates:	Date Event June 1979 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Lancashire, W. E.
Sponsors:	Griffiths, D. E.
Extent:	x, 117 leaves : illustrations, charts
Language:	eng

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