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Pattern engineering of living bacterial colonies using meniscus-driven fluidic channels
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Kantsler, Vasily, Ontañón-McDonald, Elena, Kuey, Cansu, Ghanshyam, Manjari J. , Roffin, Maria Chiara and Asally, Munehiro (2020) Pattern engineering of living bacterial colonies using meniscus-driven fluidic channels. ACS Synthetic Biology, 9 (6). pp. 1277-1283. doi:10.1021/acssynbio.0c00146 ISSN 2161-5063.
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WRAP-pattern-engineering-living-bacterial-colonies-using-meniscus-driven-fluidic-channels-Kantsler-2020.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1692Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acssynbio.0c00146
Abstract
Creating adaptive, sustainable, and dynamic biomaterials is a forthcoming mission of synthetic biology. Engineering spatially organized bacterial communities has a potential to develop such bio-metamaterials. However, generating living patterns with precision, robustness, and a low technical barrier remains as a challenge. Here we present an easily implementable technique for patterning live bacterial populations using a controlled meniscus-driven fluidics system, named as MeniFluidics. We demonstrate multiscale patterning of biofilm colonies and swarms with submillimeter resolution. Utilizing the faster bacterial spreading in liquid channels, MeniFluidics allows controlled bacterial colonies both in space and time to organize fluorescently labeled Bacillus subtilis strains into a converged pattern and to form dynamic vortex patterns in confined bacterial swarms. The robustness, accuracy, and low technical barrier of MeniFluidics offer a tool for advancing and inventing new living materials that can be combined with genetically engineered systems, and adding to fundamental research into ecological, evolutional, and physical interactions between microbes.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QC Physics Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology T Technology > TA Engineering (General). Civil engineering (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Biophysics, Soft condensed matter, Biofilms, Synthetic biology, Meniscus (Liquids) | |||||||||||||||
| Journal or Publication Title: | ACS Synthetic Biology | |||||||||||||||
| Publisher: | American Chemical Society | |||||||||||||||
| ISSN: | 2161-5063 | |||||||||||||||
| Official Date: | 3 June 2020 | |||||||||||||||
| Dates: |
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| Volume: | 9 | |||||||||||||||
| Number: | 6 | |||||||||||||||
| Page Range: | pp. 1277-1283 | |||||||||||||||
| DOI: | 10.1021/acssynbio.0c00146 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Reuse Statement (publisher, data, author rights): | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synthetic Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acssynbio.0c00146 | |||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
| Date of first compliant deposit: | 11 June 2020 | |||||||||||||||
| Date of first compliant Open Access: | 3 June 2021 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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