The Library
Fluorescent oligonucleotide rDNA probes for specific detection of methane oxidising bacteria
Tools
UNSPECIFIED (2000) Fluorescent oligonucleotide rDNA probes for specific detection of methane oxidising bacteria. FEMS MICROBIOLOGY ECOLOGY, 31 (1). pp. 29-38. ISSN 0168-6496.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
Oligonucleotide probes targeting the 16S rRNA of distinct phylogenetic groups of methanotrophs were designed for the in situ detection of these organisms. A probe, MG-64, detected specifically type I methanotrophs. while probes MA-221 and MA-621, detected type II methanotrophs in whole cell hybridisations. A probe Mc1029 was also designed which targeted only organisms from the Methylococcus genus after whole cell hybridisations. All probes were labelled with the fluorochrome Cy3 and optimum conditions for hybridisation were determined. Non-specific target sites of the type I (MG-64) and type IT (MA-621) probes to non-methanotrophic organisms are highlighted. The probes are however used in studying enrichment cultures and environments when selective pressure favours the growth of methanotrophs over other organisms. The application of these probes was demonstrated in the detection of type I methanotrophs with the MG-64 probe in an enrichment culture from an estuarine sample demonstrating methane oxidation. The detection of type I methanotrophs was confirmed by a 16S rDNA molecular analysis of the estuarine enrichment culture which demonstrated that the most abundant bacterial clone type in the 16S rDNA library was most closely related to Methylobacter sp. strain BB5.1, a type I methanotroph also isolated from an estuarine environment. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY ECOLOGY | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0168-6496 | ||||
Official Date: | January 2000 | ||||
Dates: |
|
||||
Volume: | 31 | ||||
Number: | 1 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 29-38 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |