Highfield, Peter Edmund (1978) Studies on the synthesis of the small subunit of fraction I protein. PhD thesis, University of Warwick.

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Abstract

The small subunit of Fraction I protein from pea leaves has been synthesised in vitro by translation of poly(A)- containing RNA , obtained from pea shoot polysomes, in a cell- free protein-synthesising system prepared from wheat-germ.

The products of this translation were characterised by gel electrophoretic and immunological techniques. It was found that the small subunit of Fraction I protein was synthesised as a precursor of 20,000 molecular weight; this precursor has been called P20.

In the presence of isolated, intact chloroplasts, P20 is processed to the small subunit. The small subunit so produced is resistant to trypsin-digestion and so is assumed to have entered the chloroplasts. The processing activity is apparently associated with the chloroplast envelope. A mechanism for this processing and transport is proposed and is discussed in the light of other models currently available for the transport of proteins across membranes.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QD Chemistry Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Proteins -- Synthesis, Proteins -- Separation, Peas -- Anatomy, Wheat germ
Official Date:	February 1978
Dates:	Date Event February 1978 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Ellis, R. J. (Reginald John)
Extent:	268, [26] leaves : illustrations
Language:	eng

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