The Library
METHYLTRANSFERASE1 and ripening modulate vivipary during tomato fruit development
Tools
Yao, Mengqin, Chen, Wei Wei, Kong, Junhua, Zhang, Xinlian, Shi, Nongnong, Zhong, Silin, Ma, Ping, Gallusci, Philippe, Jackson, Stephen D. , Liu, Yule and Hong, Yiguo (2020) METHYLTRANSFERASE1 and ripening modulate vivipary during tomato fruit development. Plant Physiology, 183 (4). pp. 1883-1897. doi:10.1104/pp.20.00499 ISSN 0032-0889.
PDF
WRAP-SlMET1-ripening-vivipary-Jackson-2020.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (2639Kb) |
Official URL: http://dx.doi.org/10.1104/pp.20.00499
Abstract
Vivipary, wherein seeds germinate prior to dispersal while still associated with the maternal plant, is an adaptation to extreme environments. It is normally inhibited by the establishment of dormancy. The genetic framework of vivipary has been well studied; however, the role of epigenetics in vivipary remains unknown. Here, we report that silencing of METHYLTRANSFERASE1 (SlMET1) promoted precocious seed germination and seedling growth within the tomato (Solanum lycopersicum) epimutant Colourless non-ripening (Cnr) fruits. This was associated with decreases in abscisic acid (ABA) concentration and levels of mRNA encoding 9-cis-epoxycarotenoid-dioxygenase (SlNCED), which is involved in ABA biosynthesis. Differentially methylated regions were identified in promoters of differentially expressed genes, including SlNCED. SlNCED knockdown also induced viviparous seedling growth in Cnr fruits. Strikingly, Cnr ripening reversion suppressed vivipary. Moreover, neither SlMET1/SlNCED-VIGS nor transgenic SlMET1-RNAi produced vivipary in wild-type tomatoes; the latter affected leaf architecture, arrested flowering and repressed seed development. Thus, a dual pathway in ripening and SlMET1-mediated epigenetics coordinates the blockage of seed vivipary.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Plant Physiology | ||||||
Publisher: | American Society of Plant Biologists | ||||||
ISSN: | 0032-0889 | ||||||
Official Date: | 5 June 2020 | ||||||
Dates: |
|
||||||
Volume: | 183 | ||||||
Number: | 4 | ||||||
Page Range: | pp. 1883-1897 | ||||||
DOI: | 10.1104/pp.20.00499 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Copyright Holders: | Copyright © 2020 American Society of Plant Biologists | ||||||
Date of first compliant deposit: | 19 June 2020 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |