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Exploring the lignin catabolism potential of soil-derived lignocellulolytic microbial consortia by a gene-centric metagenomic approach
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Díaz-García, Laura, Bugg, Timothy D. H. and Jiménez, Diego Javier (2020) Exploring the lignin catabolism potential of soil-derived lignocellulolytic microbial consortia by a gene-centric metagenomic approach. Microbial Ecology, 80 . 885-896 . doi:10.1007/s00248-020-01546-1 ISSN 0095-3628.
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WRAP-Exploring-lignin-catabolism-potential-soil-lignocellulolytic-microbial-consortia-gene-centric-metagenomic-2020.pdf - Accepted Version - Requires a PDF viewer. Download (1944Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s00248-020-01546-1
Abstract
An exploration of the ligninolytic potential of lignocellulolytic microbial consortia can improve our understanding of the eco-enzymology of lignin conversion in nature. In this study, we aimed to detect enriched lignin-transforming enzymes on metagenomes from three soil-derived microbial consortia that were cultivated on “pre-digested” plant biomass (wheat straw, WS1-M; switchgrass, SG-M; and corn stover, CS-M). Of 60 selected enzyme-encoding genes putatively involved in lignin catabolism, 20 genes were significantly abundant in WS1-M, CS-M, and/or SG-M consortia compared with the initial forest soil inoculum metagenome (FS1). These genes could be involved in lignin oxidation (e.g., superoxide dismutases), oxidative stress responses (e.g., catalase/peroxidases), generation of protocatechuate (e.g., vanAB genes), catabolism of gentisate, catechol and 3-phenylpropionic acid (e.g., gentisate 1,2-dioxygenases, muconate cycloisomerases, and hcaAB genes), the beta-ketoadipate pathway (e.g., pcaIJ genes), and tolerance to lignocellulose-derived inhibitors (e.g., thymidylate synthases). The taxonomic affiliation of 22 selected lignin-transforming enzymes from WS1-M and CS-M consortia metagenomes revealed that Pseudomonadaceae, Alcaligenaceae, Sphingomonadaceae, Caulobacteraceae, Comamonadaceae, and Xanthomonadaceae are the key bacterial families in the catabolism of lignin. A predictive “model” was sketched out, where each microbial population has the potential to metabolize an array of aromatic compounds through different pathways, suggesting that lignin catabolism can follow a “task division” strategy. Here, we have established an association between functions and taxonomy, allowing a better understanding of lignin transformations in soil-derived lignocellulolytic microbial consortia, and pinpointing some bacterial taxa and catabolic genes as ligninolytic trait-markers.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QH Natural history T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||
Library of Congress Subject Headings (LCSH): | Lignocellulose, Lignocellulose -- Biodegradation, Lignin , Metagenomics | |||||||||
Journal or Publication Title: | Microbial Ecology | |||||||||
Publisher: | Springer | |||||||||
ISSN: | 0095-3628 | |||||||||
Official Date: | November 2020 | |||||||||
Dates: |
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Volume: | 80 | |||||||||
Page Range: | 885-896 | |||||||||
DOI: | 10.1007/s00248-020-01546-1 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Reuse Statement (publisher, data, author rights): | This is a post-peer-review, pre-copyedit version of an article published in Microbial Ecology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00248-020-01546-1 | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Date of first compliant deposit: | 29 June 2020 | |||||||||
Date of first compliant Open Access: | 22 June 2021 | |||||||||
RIOXX Funder/Project Grant: |
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