The Library
Rotavirus group : a genotype circulation patterns across Kenya before and after nationwide vaccine introduction, 2010-2018
Tools
(2020) Rotavirus group : a genotype circulation patterns across Kenya before and after nationwide vaccine introduction, 2010-2018. BMC Infectious Diseases, 20 . 504. doi:10.1186/s12879-020-05230-0 ISSN 1471-2334.
|
PDF
WRAP-Rotavirus-group-A-genotype-circulation-Nokes-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1722Kb) | Preview |
|
PDF
WRAP-Rotavirus-group-A-genotype-circulation-patterns-across-Kenya-Nokes-2020.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (2743Kb) |
Official URL: https://doi.org/10.1186/s12879-020-05230-0
Abstract
Background
Kenya introduced the monovalent G1P [8] Rotarix® vaccine into the infant immunization schedule in July 2014. We examined trends in rotavirus group A (RVA) genotype distribution pre- (January 2010–June 2014) and post- (July 2014–December 2018) RVA vaccine introduction.
Methods
Stool samples were collected from children aged < 13 years from four surveillance sites across Kenya: Kilifi County Hospital, Tabitha Clinic Nairobi, Lwak Mission Hospital, and Siaya County Referral Hospital (children aged < 5 years only). Samples were screened for RVA using enzyme linked immunosorbent assay (ELISA) and VP7 and VP4 genes sequenced to infer genotypes.
Results
We genotyped 614 samples in pre-vaccine and 261 in post-vaccine introduction periods. During the pre-vaccine introduction period, the most frequent RVA genotypes were G1P [8] (45.8%), G8P [4] (15.8%), G9P [8] (13.2%), G2P [4] (7.0%) and G3P [6] (3.1%). In the post-vaccine introduction period, the most frequent genotypes were G1P [8] (52.1%), G2P [4] (20.7%) and G3P [8] (16.1%). Predominant genotypes varied by year and site in both pre and post-vaccine periods. Temporal genotype patterns showed an increase in prevalence of vaccine heterotypic genotypes, such as the commonly DS-1-like G2P [4] (7.0 to 20.7%, P < .001) and G3P [8] (1.3 to 16.1%, P < .001) genotypes in the post-vaccine introduction period. Additionally, we observed a decline in prevalence of genotypes G8P [4] (15.8 to 0.4%, P < .001) and G9P [8] (13.2 to 5.4%, P < .001) in the post-vaccine introduction period. Phylogenetic analysis of genotype G1P [8], revealed circulation of strains of lineages G1-I, G1-II and P [8]-1, P [8]-III and P [8]-IV. Considerable genetic diversity was observed between the pre and post-vaccine strains, evidenced by distinct clusters.
Conclusion
Genotype prevalence varied from before to after vaccine introduction. Such observations emphasize the need for long-term surveillance to monitor vaccine impact. These changes may represent natural secular variation or possible immuno-epidemiological changes arising from the introduction of the vaccine. Full genome sequencing could provide insights into post-vaccine evolutionary pressures and antigenic diversity.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Rotaviruses, Rotavirus infections -- Kenya -- Kilifi District, Rotavirus infections -- Vaccination, Viral vaccines, Gastroenteritis in children | ||||||
Journal or Publication Title: | BMC Infectious Diseases | ||||||
Publisher: | BioMed Central Ltd. | ||||||
ISSN: | 1471-2334 | ||||||
Official Date: | 13 July 2020 | ||||||
Dates: |
|
||||||
Volume: | 20 | ||||||
Article Number: | 504 | ||||||
DOI: | 10.1186/s12879-020-05230-0 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 7 July 2020 | ||||||
Date of first compliant Open Access: | 20 July 2020 | ||||||
RIOXX Funder/Project Grant: |
|
||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year