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Data for Post-thaw culture and measurement of total cell recovery is crucial in the evaluation of new macromolecular cryoprotectants
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Murray, Kathryn and Gibson, Matthew I. (2020) Data for Post-thaw culture and measurement of total cell recovery is crucial in the evaluation of new macromolecular cryoprotectants. [Dataset]
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Abstract
The storage and transport of cells is a fundamental technology which underpins cell biology, biomaterials research, and emerging cell-based therapies. Inspired by antifreeze and ice-binding proteins in extremophiles, macromolecular (polymer) cryoprotectants are emerging as exciting biomaterials to enable the reduction and/or replacement of conventional cryoprotective agents such as DMSO. Here, we critically study post-thaw cellular outcomes upon addition of macromolecular cryoprotectants to provide unambiguous evidence that post-thaw culturing time and a mixture of assays are essential to claim a positive outcome. In particular, we observe that only measuring the viability of recovered cells gives false positives, even with non-cryoprotective polymers. Several systems gave apparently high viability but very low total cell recovery, which could be reported as a success but in practical applications would not be useful. Post-thaw culture time is also shown to be crucial to enable apoptosis to set in. Using this approach we demonstrate that polyampholytes (a rapidly emerging class of cryoprotectants) improve post-thaw outcomes across both measures, compared to poly(ethylene glycol), which can give false positives when only viability and short post-thaw time scales are considered. This work will help guide the discovery of new macromolecular cryoprotectants and ensure materials which only give positive results under limited outcomes can be quickly identified and removed.
Item Type: | Dataset | ||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||
Type of Data: | Experimental data | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Macromolecules -- Research, Cytology -- Research, Cryobiology | ||||||||||||||||||
Publisher: | University of Warwick, Department of Chemistry | ||||||||||||||||||
Official Date: | 8 July 2020 | ||||||||||||||||||
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Status: | Not Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Media of Output (format): | .xlsx .rtf | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Copyright Holders: | University of Warwick | ||||||||||||||||||
Description: | Data record consists of a zip archive containing data in .xlsx format and an accompanying index file. Further information for the specific data files can be found in the index. |
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Date of first compliant deposit: | 8 July 2020 | ||||||||||||||||||
Date of first compliant Open Access: | 8 July 2020 | ||||||||||||||||||
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