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Pad-printed Prussian blue doped carbon ink for real-time peroxide sensing in cell culture
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McBeth, Craig, Paterson, Andrew H. and Sharp, Duncan (2020) Pad-printed Prussian blue doped carbon ink for real-time peroxide sensing in cell culture. Journal of Electroanalytical Chemistry, 878 . doi:10.1016/j.jelechem.2020.114537 ISSN 1572-6657.
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WRAP-pad-printed-Prussian-blue-doped-carbon-ink-real-time-peroxide-sensing-cell-culture-McBeth-2020.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (2177Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.jelechem.2020.114537
Abstract
Hydrogen peroxide has important roles within cellular functions, as a prevalent form of Reactive Oxygen Species, detection within mammalian cells is of metabolic importance; typically requiring cell lysis or fluorescence-based methods to quantify.
Herein, we explore the novel use of Prussian blue mediated, pad printed carbon electrodes to allow the indirect detection of cellular peroxides in bulk culture media, which facilitates non-invasive, real-time detection.
Electrodes demonstrated capacity to detect H2O2 with a linear range of 1-200 μM in CMEM (R2 = 0.9988), enabling detection of peroxides found in culture media and lysate. Developed electrodes had a Limit of Detection (LOD) of 0.41 μM H2O2 in Britton-Robinson Buffer (BRB), 0.38 μM in Eagle's Minimum Essential Medium (EMEM) and 9.19 μM in Dulbecco's Modified Eagles Medium (DMEM). Electrodes were tested in a conventional 5% serum supplemented EMEM (CMEM) and demonstrated an LOD of 0.5 μM and LOQ of 0.9 μM.
The results demonstrate proof of concept for monitoring H2O2 in complex culture media with potential long-term use and reusability using simple, pad printed Prussian Blue / Carbon electrodes. The lack of further modification, and cost-effectiveness of these disposable electrodes could offer great advancement to monitoring of peroxides in complex media.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Prussian blue, Cell culture , Electrodes, Carbon, Hydrogen peroxide | ||||||||
Journal or Publication Title: | Journal of Electroanalytical Chemistry | ||||||||
Publisher: | Elsevier Science SA | ||||||||
ISSN: | 1572-6657 | ||||||||
Official Date: | 1 December 2020 | ||||||||
Dates: |
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Volume: | 878 | ||||||||
Number of Pages: | 114537 | ||||||||
DOI: | 10.1016/j.jelechem.2020.114537 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 25 August 2020 | ||||||||
Date of first compliant Open Access: | 7 August 2021 |
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