Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Engineering isoprenoid quinone production in yeast

Tools
- Tools
+ Tools

Kaur, Divjot, Alkhder, Duha, Corre, Christophe and Alberti, Fabrizio (2020) Engineering isoprenoid quinone production in yeast. ACS Synthetic Biology, 9 (9). pp. 2239-2245. doi:10.1021/acssynbio.0c00081 ISSN 2161-5063.

[img]
Preview
PDF
WRAP-Engineering-isoprenoid-quinone-production-yeast-Kaur-2020.pdf - Accepted Version - Requires a PDF viewer.

Download (1720Kb) | Preview
[img]
Preview
PDF
WRAP-Supplementary-2020.pdf - Supplemental Material - Requires a PDF viewer.

Download (2657Kb) | Preview
Official URL: https://doi.org/10.1021/acssynbio.0c00081

Request Changes to record.

Abstract

Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker’s yeast strain, Saccharomyces cerevisiae EPYFA3, for the production of isoprenoid quinones. Our yeast strain was developed through overexpression of the shikimate pathway in a well-established recipient strain (S. cerevisiae EPY300) where the mevalonate pathway is overexpressed. As a proof of concept, our new host strain was used to overproduce the endogenous isoprenoid quinone coenzyme Q6, resulting in a nearly 3-fold production increase. EPYFA3 represents a valuable platform for the heterologous production of high value isoprenoid quinones. EPYFA3 will also facilitate the elucidation of isoprenoid quinone biosynthetic pathways.

Item Type: Journal Article
Subjects: Q Science > QK Botany
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Isopentenoids, Ubiquinones, Yeast -- Genetic aspects, Saccharomyces cerevisiae -- Genetic aspects, Shikimic acid
Journal or Publication Title: ACS Synthetic Biology
Publisher: American Chemical Society
ISSN: 2161-5063
Official Date: 20 August 2020
Dates:
DateEvent
20 August 2020Published
12 August 2020Available
12 August 2020Accepted
Volume: 9
Number: 9
Page Range: pp. 2239-2245
DOI: 10.1021/acssynbio.0c00081
Status: Peer Reviewed
Publication Status: Published
Reuse Statement (publisher, data, author rights): This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synthetic Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acssynbio.0c00081
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 1 September 2020
Date of first compliant Open Access: 12 August 2021
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
ECF-2018-691Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
EP/L016494/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us