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Framework for quantification of the dynamics of root colonization by pseudomonas fluorescens isolate SBW25
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Carroll, Daire, Holden, Nicola, Gifford, Miriam L. and Dupuy, Lionel X. (2020) Framework for quantification of the dynamics of root colonization by pseudomonas fluorescens isolate SBW25. Frontiers in Microbiology, 11 . doi:10.3389/fmicb.2020.585443 ISSN 1664-302X.
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WRAP-Framework-quantification-dynamics-root-colonization-fluorescens-isolate-SBW25-Gifford-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2452Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fmicb.2020.585443
Abstract
Colonization of the root surface, or rhizoplane, is one of the first steps for soil-borne bacteria to become established in the plant microbiome. However, the relative contributions of processes, such as bacterial attachment and proliferation is not well characterized, and this limits our ability to comprehend the complex dynamics of microbial communities in the rhizosphere. The work presented here addresses this knowledge gap. A model system was developed to acquire quantitative data on the colonization process of lettuce (Lactuca sativa L. cultivar. All Year Round) roots by Pseudomonas fluorescens isolate SBW25. A theoretical framework is proposed to calculate attachment rate and quantify the relative contribution of bacterial attachment to colonization. This allows the assessment of attachment rates on the root surface beyond the short time period during which it can be quantified experimentally. All techniques proposed are generic and similar analyses could be applied to study various combinations of plants and bacteria, or to assess competition between species. In the future this could allow for selection of microbial traits that improve early colonization and maintenance of targeted isolates in cropping systems, with potential applications for the development of biological fertilizers.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Rhizosphere, Pseudomonas fluorescens, Soil ecology | |||||||||||||||
Journal or Publication Title: | Frontiers in Microbiology | |||||||||||||||
Publisher: | Frontiers Research Foundation | |||||||||||||||
ISSN: | 1664-302X | |||||||||||||||
Official Date: | 25 September 2020 | |||||||||||||||
Dates: |
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Volume: | 11 | |||||||||||||||
DOI: | 10.3389/fmicb.2020.585443 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 2 October 2020 | |||||||||||||||
Date of first compliant Open Access: | 6 October 2020 | |||||||||||||||
RIOXX Funder/Project Grant: |
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