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Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties
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Alobaid, Meshal A., Richards, Sarah-Jane, Alexander, Morgan R., Gibson, Matthew I. and Ghaemmaghami, Amir M. (2020) Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties. Materials Today Bio, 8 . 100080. doi:10.1016/j.mtbio.2020.100080 ISSN 2590-0064.
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WRAP-Developing-immune-regulatory-materials-immobilized-monosaccharides-Gibson-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2819Kb) | Preview |
Official URL: https://doi.org/10.1016/j.mtbio.2020.100080
Abstract
New strategies for immune modulation have shown real promise in regenerative medicine as well as the fight against autoimmune diseases, allergies and cancer. Dendritic cells (DCs) are gatekeepers of the immune system and their ability in shaping the adaptive immune responses makes DCs ideal targets for immune modulation. Carbohydrates are abundant in different biological systems and are known to modulate DC phenotype and function. However, how simple monosaccharides instruct DC function is less well understood. In this study we used a combinatorial array of immobilized monosaccharides to investigate how they modulate DC phenotype and function, and crucially the impact of such changes on downstream adaptive immune responses. Our data show a selection of monosaccharides significantly suppress lipopolysaccharide (LPS) induced DC activation as evidenced by reduction in CD40 expression, IL-12 production and 2,3 indoleamine dioxygenase activity, while inducing a significant increase in IL-10 production. These changes are indicative of induction of an anti-inflammatory or regulatory phenotype in DCs which was further confirmed in DC-T cell co-cultures where DCs cultured on the ‘regulatory’ monosaccharaide coated surfaces were shown to induce naïve T cell polarization towards regulatory phenotype. Our data also highlighted a selection of monosaccharides that are able to promote mixed Treg and Th17 cell differentiation, a T cell phenotype expected to be highly immune suppressive. These data show the potential immunomodulatory effects of immobilized monosaccharides in priming DCs and skewing T cell differentiation towards an immune-regulatory phenotype. The ability to fine tune immune responses using these simple carbohydrate combinations (e.g. as coatings for existing materials) can be utilized as novel tools for immune modulation with potential applications in regenerative medicine, implantable medical devices and wound healing where reduction of inflammatory responses and maintaining immune homeostasis are desirable.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Dendritic cells, T cells, Immune response -- Regulation, Galactose, Mannose | |||||||||
Journal or Publication Title: | Materials Today Bio | |||||||||
Publisher: | Elsevier | |||||||||
ISSN: | 2590-0064 | |||||||||
Official Date: | September 2020 | |||||||||
Dates: |
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Volume: | 8 | |||||||||
Article Number: | 100080 | |||||||||
DOI: | 10.1016/j.mtbio.2020.100080 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 9 November 2020 | |||||||||
Date of first compliant Open Access: | 10 November 2020 | |||||||||
RIOXX Funder/Project Grant: |
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