The Library
Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes
Tools
Duchi, Diego, Gryte, Kristofer, Robb, Nicole C., Morichaud, Zakia, Sheppard, Carol, Brodolin, Konstantin, Wigneshweraraj, Sivaramesh and Kapanidis, Achillefs N (2018) Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes. Nucleic Acids Research, 46 (2). pp. 677-688. doi:10.1093/nar/gkx1146 ISSN 0305-1048.
|
PDF
WRAP-conformational-heterogeneity-bubble-dynamics-single-bacterial-transcription-initiation-complexes-Robb-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (4Mb) | Preview |
Official URL: http://dx.doi.org/10.1093/nar/gkx1146
Abstract
Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and an RNAP-promoter open complex; however, the series and sequence of conformational changes, and the factors that influence them are unclear. To address the conformational landscape and transitions in transcription initiation, we applied single-molecule Förster resonance energy transfer (smFRET) on immobilized Escherichia coli transcription open complexes. Our results revealed the existence of two stable states within RNAP–DNA complexes in which the promoter DNA appears to adopt closed and partially open conformations, and we observed large-scale transitions in which the transcription bubble fluctuated between open and closed states; these transitions, which occur roughly on the 0.1 s timescale, are distinct from the millisecond-timescale dynamics previously observed within diffusing open complexes. Mutational studies indicated that the σ70 region 3.2 of the RNAP significantly affected the bubble dynamics. Our results have implications for many steps of transcription initiation, and support a bend-load-open model for the sequence of transitions leading to bubble opening during open complex formation.
Item Type: | Journal Article | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Genetic regulation, RNA polymerase, Energy transfer , Gene expression, Genetic transcription , Genetic transcription -- Regulation | ||||||||||||||||||
Journal or Publication Title: | Nucleic Acids Research | ||||||||||||||||||
Publisher: | Oxford University Press | ||||||||||||||||||
ISSN: | 0305-1048 | ||||||||||||||||||
Official Date: | 25 January 2018 | ||||||||||||||||||
Dates: |
|
||||||||||||||||||
Volume: | 46 | ||||||||||||||||||
Number: | 2 | ||||||||||||||||||
Page Range: | pp. 677-688 | ||||||||||||||||||
DOI: | 10.1093/nar/gkx1146 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 15 October 2020 | ||||||||||||||||||
Date of first compliant Open Access: | 16 October 2020 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year