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A plastidial lysophosphatidic acid acyltransferase from oilseed rape
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UNSPECIFIED (1999) A plastidial lysophosphatidic acid acyltransferase from oilseed rape. PLANT PHYSIOLOGY, 120 (3). pp. 913-921. ISSN 0032-0889.
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Abstract
The biosynthesis of phosphatidic acid, a key intermediate in the biosynthesis of lipids, is controlled by lysophosphatidic acid (LPA, or 1-acyl-glycerol-3-P) acyltransferase (LPAAT, EC 2.3.1.51). We have isolated a cDNA encoding a novel LPAAT by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape (Brassica napus). Transformation of the acyltransferase-deficient E. coli strain JC201 with the cDNA sequence BAT2 alleviated the temperature-sensitive phenotype of the plsC mutant and conferred a palmitoyl-coenzyme A-preferring acyltransferase activity to membrane fractions. The BAT2 cDNA encoded a protein of 351 amino acids with a predicted molecular mass of 38 kD and an isoelectric point of 9.7. Chloroplast-import experiments showed processing of a BAT2 precursor protein to a mature protein of approximately 32 kD, which was localized in the membrane fraction. BAT2 is encoded by a minimum of two genes that may be expressed ubiquitously. These data are consistent with the identity of BAT2 as the plastidial enzyme of the prokaryotic glycerol-3-P pathway that uses a palmitoyl-ACP to produce phosphatidic acid with a prokaryotic-type acyl composition. The homologies between the deduced protein sequence of BAT2 with prokaryotic and eukaryotic microsomal LAP acytransferases suggest that seed microsomal forms may have evolved from the plastidial enzyme.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Journal or Publication Title: | PLANT PHYSIOLOGY | ||||
Publisher: | AMER SOC PLANT PHYSIOLOGISTS | ||||
ISSN: | 0032-0889 | ||||
Official Date: | July 1999 | ||||
Dates: |
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Volume: | 120 | ||||
Number: | 3 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 913-921 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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