Sagar, Nidhi M., Duboc, Henri, Kay, Gemma L., Alam, Mohammad T., Wicaksono, Alfian, Covington, James A., Quince, Christopher , Kokkorou, Margarita, Svolos, Vaios, Palmieri, Lola J., Gerasimidis, Konstantinos, Walters, Julian R. F. and Arasaradnam, Ramesh P. (2020) The pathophysiology of bile acid diarrhoea : differences in the colonic microbiome, metabolome and bile acids. Scientific Reports, 10 (1). 20436. doi:10.1038/s41598-020-77374-7 ISSN 2045-2322.

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Abstract

Bile acid diarrhoea (BAD) is a common disorder resulting from increased loss of bile acids (BAs), overlapping irritable bowel syndrome with diarrhoea (IBS-D). The gut microbiota metabolises primary BAs to secondary BAs, with differing impacts on metabolism and homeostasis. The aim of this study was to profile the microbiome, metabolic products and bile acids in BAD. Patients with BAD diagnosed by SeHCAT testing, were compared with other IBS-D patients, and healthy controls. Faecal 16S ribosomal RNA gene analysis was undertaken. Faecal short chain fatty acid (SCFA) and urinary volatile organic compounds (VOCs) were measured. BAs were quantified in serum and faeces. Faecal bacterial diversity was significantly reduced in patients with BAD. Several taxa were enriched compared to IBS-D. SCFA amounts differed in BAD, controls and IBS-D, with significantly more propionate in BAD. Separation of VOC profiles was evident, but the greatest discrimination was between IBS-D and controls. Unconjugated and primary BA in serum and faeces were significantly higher in BAD. The faecal percentage primary BA was inversely related to SeHCAT. BAD produces dysbiosis, with metabolite differences, including VOC, SCFA and primary BAs when compared to IBS-D. These findings provide new mechanistic insights into the pathophysiology of BAD.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RC Internal medicine
Divisions:	Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor:	Library Publications Router
Library of Congress Subject Headings (LCSH):	Diarrhea, Bile acids, Gastrointestinal system -- Microbiology, Intestines -- Diseases
Journal or Publication Title:	Scientific Reports
Publisher:	Nature Publishing Group UK
ISSN:	2045-2322
Official Date:	24 November 2020
Dates:	Date Event 24 November 2020 Available 27 October 2020 Accepted
Volume:	10
Number:	1
Article Number:	20436
DOI:	10.1038/s41598-020-77374-7
Status:	Peer Reviewed
Publication Status:	Published
Reuse Statement (publisher, data, author rights):	** From Springer Nature via Jisc Publications Router ** History: received 25-08-2020; accepted 27-10-2020; registration 11-11-2020; online 24-11-2020; pub-electronic 24-11-2020; collection 12-2020. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	7 December 2020
Date of first compliant Open Access:	7 December 2020
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED University Hospitals Coventry and Warwick Trust Charity https://www.uhcw.nhs.uk/our-services-and-people/uhcw-charity/ UNSPECIFIED Midlands Gastroenterology Society UNSPECIFIED UNSPECIFIED Bardhan Research and Education Trust https://www.thebret.org/
Related URLs:	http://creativecommons.org/licenses/by/4...

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