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Engineering bacteria to produce pure phage-like particles for gene delivery
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Tridgett, Matthew, Ababi, Maria, Osgerby, Alexander, Ramirez Garcia, Robert and Jaramillo, Alfonso (2021) Engineering bacteria to produce pure phage-like particles for gene delivery. ACS Synthetic Biology, 10 (1). pp. 107-114. doi:10.1021/acssynbio.0c00467 ISSN 2161-5063.
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Official URL: https://doi.org/10.1021/acssynbio.0c00467
Abstract
Natural and engineered phages have been used in many applications, but their use to deliver user-defined genetic cargoes has been hampered by contamination with replicative phage, restricting use of the technology beyond the laboratory. Here we present a method to produce transducing particles without contamination. In addition, we demonstrate the use of a helper phage-free transducing particle preparation as an antimicrobial agent. This will pave the way for the development of new phage-based technologies with greater scope than lytic phage therapy.
| Item Type: | Journal Article | ||||||||||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | ACS Synthetic Biology | ||||||||||||||||||
| Publisher: | American Chemical Society | ||||||||||||||||||
| ISSN: | 2161-5063 | ||||||||||||||||||
| Official Date: | 15 January 2021 | ||||||||||||||||||
| Dates: |
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| Volume: | 10 | ||||||||||||||||||
| Number: | 1 | ||||||||||||||||||
| Page Range: | pp. 107-114 | ||||||||||||||||||
| DOI: | 10.1021/acssynbio.0c00467 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
| Description: | |||||||||||||||||||
| Date of first compliant deposit: | 4 December 2020 | ||||||||||||||||||
| Date of first compliant Open Access: | 22 December 2020 | ||||||||||||||||||
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