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Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research
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Alberti, Fabrizio, Kaleem, Saraa and Weaver, Jack (2020) Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research. Biology Open, 9 (12). bio056010. doi:10.1242/bio.056010 ISSN 2046-6390.
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WRAP-recent-developements-basidiomycete-product-research-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (809Kb) | Preview |
Official URL: https://doi.org/10.1242/bio.056010
Abstract
Basidiomycota are a large and diverse phylum of fungi. They can make bioactive metabolites that are used or have inspired the synthesis of antibiotics and agrochemicals. Terpenoids are the most abundant class of natural products encountered in this taxon. Other natural product classes have been described, including polyketides, peptides, and indole alkaloids. The discovery and study of natural products made by basidiomycete fungi has so far been hampered by several factors, which include their slow growth and complex genome architecture. Recent developments of tools for genome and metabolome studies are allowing researchers to more easily tackle the secondary metabolome of basidiomycete fungi. Inexpensive long-read whole-genome sequencing enables the assembly of high-quality genomes, improving the scaffold upon which natural product gene clusters can be predicted. CRISPR/Cas9-based engineering of basidiomycete fungi has been described and will have an important role in linking natural products to their genetic determinants. Platforms for the heterologous expression of basidiomycete genes and gene clusters have been developed, enabling natural product biosynthesis studies. Molecular network analyses and publicly available natural product databases facilitate data dereplication and natural product characterisation. These technological advances combined are prompting a revived interest in natural product discovery from basidiomycete fungi.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Basidiomycota -- Metabolism, Basidiomycota -- Genetic aspects, Fungal metabolites, CRISPR-associated protein 9 | |||||||||||||||
| Journal or Publication Title: | Biology Open | |||||||||||||||
| Publisher: | The Company of Biologists Ltd. | |||||||||||||||
| ISSN: | 2046-6390 | |||||||||||||||
| Official Date: | 2 December 2020 | |||||||||||||||
| Dates: |
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| Volume: | 9 | |||||||||||||||
| Number: | 12 | |||||||||||||||
| Article Number: | bio056010 | |||||||||||||||
| DOI: | 10.1242/bio.056010 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 8 December 2020 | |||||||||||||||
| Date of first compliant Open Access: | 9 December 2020 | |||||||||||||||
| Funder: | The Leverhulme Trust [ECF- 2018-691] and the University of Warwick, through an Early Career Fellowship to F.A.; the Engineering and Physical Sciences Research Council and the Biotechnology and Biological Sciences Research Council [EP/L016494/1] for a scho | |||||||||||||||
| RIOXX Funder/Project Grant: |
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