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Scanning ion conductance microscopy reveals differences in the ionic environments of gram-positive and negative bacteria
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Cremin, Kelsey , Jones, Bryn, Teahan, James, Meloni, Gabriel N., Perry, David, Zerfass, Christian, Asally, Munehiro, Soyer, Orkun S. and Unwin, Patrick R. (2020) Scanning ion conductance microscopy reveals differences in the ionic environments of gram-positive and negative bacteria. Analytical Chemistry, 92 (24). pp. 16024-16032. doi:10.1021/acs.analchem.0c03653
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Official URL: https://doi.org/10.1021/acs.analchem.0c03653
Abstract
This paper reports on the use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge environment of two live bacterial strains: the Gram-negative and the Gram-positive . SICM results find heterogeneities across the bacterial surface and significant differences among the Gram-positive and Gram-negative bacteria. The bioelectrical environment of the was found to be considerably more negatively charged compared to . SICM measurements, fitted to a simplified finite element method (FEM) model, revealed surface charge values of -80 to -140 mC m for the Gram-negative . The Gram-positive show a much higher conductivity around the cell wall, and surface charge values between -350 and -450 mC m were found using the same simplified model. SICM was also able to detect regions of high negative charge near , not detected in the topographical SICM response and attributed to the extracellular polymeric substance. To further explore how the cell wall structure can influence the SICM current response, a more comprehensive FEM model, accounting for the physical properties of the Gram-positive cell wall, was developed. The new model provides a more realistic description of the cell wall and allows investigation of the relation between its key properties and SICM currents, building foundations to further investigate and improve understanding of the Gram-positive cellular microenvironment.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- ) |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Scanning probe microscopy , Ion channels , Gram-negative bacteria, Gram-positive bacteria | |||||||||||||||||||||
| Journal or Publication Title: | Analytical Chemistry | |||||||||||||||||||||
| Publisher: | American Chemical Society | |||||||||||||||||||||
| ISSN: | 0003-2700 | |||||||||||||||||||||
| Official Date: | 15 December 2020 | |||||||||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 22 December 2020 | |||||||||||||||||||||
| Volume: | 92 | |||||||||||||||||||||
| Number: | 24 | |||||||||||||||||||||
| Page Range: | pp. 16024-16032 | |||||||||||||||||||||
| DOI: | 10.1021/acs.analchem.0c03653 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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