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Scanning ion conductance microscopy reveals differences in the ionic environments of gram-positive and negative bacteria

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Cremin, Kelsey , Jones, Bryn, Teahan, James, Meloni, Gabriel N., Perry, David, Zerfass, Christian, Asally, Munehiro, Soyer, Orkun S. and Unwin, Patrick R. (2020) Scanning ion conductance microscopy reveals differences in the ionic environments of gram-positive and negative bacteria. Analytical Chemistry, 92 (24). pp. 16024-16032. doi:10.1021/acs.analchem.0c03653 ISSN 0003-2700.

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Official URL: https://doi.org/10.1021/acs.analchem.0c03653

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Abstract

This paper reports on the use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge environment of two live bacterial strains: the Gram-negative and the Gram-positive . SICM results find heterogeneities across the bacterial surface and significant differences among the Gram-positive and Gram-negative bacteria. The bioelectrical environment of the was found to be considerably more negatively charged compared to . SICM measurements, fitted to a simplified finite element method (FEM) model, revealed surface charge values of -80 to -140 mC m for the Gram-negative . The Gram-positive show a much higher conductivity around the cell wall, and surface charge values between -350 and -450 mC m were found using the same simplified model. SICM was also able to detect regions of high negative charge near , not detected in the topographical SICM response and attributed to the extracellular polymeric substance. To further explore how the cell wall structure can influence the SICM current response, a more comprehensive FEM model, accounting for the physical properties of the Gram-positive cell wall, was developed. The new model provides a more realistic description of the cell wall and allows investigation of the relation between its key properties and SICM currents, building foundations to further investigate and improve understanding of the Gram-positive cellular microenvironment.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QR Microbiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Scanning probe microscopy , Ion channels , Gram-negative bacteria, Gram-positive bacteria
Journal or Publication Title: Analytical Chemistry
Publisher: American Chemical Society
ISSN: 0003-2700
Official Date: 15 December 2020
Dates:
DateEvent
15 December 2020Published
26 November 2020Available
16 November 2020Accepted
Volume: 92
Number: 24
Page Range: pp. 16024-16032
DOI: 10.1021/acs.analchem.0c03653
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Copyright Holders: Copyright © 2020 American Chemical Society
Date of first compliant deposit: 22 December 2020
Date of first compliant Open Access: 5 January 2021
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
EP/L015307/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
iCASE award[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
iCASE awardUnileverhttp://dx.doi.org/10.13039/100007190
790615Horizon 2020 Framework Programmehttp://dx.doi.org/10.13039/100010661
Wolfson Research Merit AwardRoyal Societyhttp://dx.doi.org/10.13039/501100000288
BB/S506783/1UK Research and Innovationhttp://dx.doi.org/10.13039/100014013

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