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Antibiotic efficacy testing in an ex vivo model of Pseudomonas aeruginosa and Staphylococcus aureus biofilms in the Cystic Fibrosis lung
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Harrington, Niamh, Sweeney, Esther, Alav, Ilyas, Allen, Freya, Moat, John and Harrison, Freya (2021) Antibiotic efficacy testing in an ex vivo model of Pseudomonas aeruginosa and Staphylococcus aureus biofilms in the Cystic Fibrosis lung. Journal of Visualized Experiments (167). e62187. doi:10.3791/62187 ISSN 1940-087X.
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Official URL: http://dx.doi.org/10.3791/62187
Abstract
The effective prescription of antibiotics for the bacterial biofilms present within the lungs of individuals with cystic fibrosis (CF) is limited by a poor correlation between antibiotic susceptibility testing (AST) results using standard diagnostic methods (e.g., broth microdilution, disk diffusion, or Etest) and clinical outcomes after antibiotic treatment. Attempts to improve AST by the use of off-the-shelf biofilm growth platforms show little improvement in results. The limited ability of in vitro biofilm systems to mimic the physicochemical environment of the CF lung and, therefore bacterial physiology
and biofilm architecture, also acts as a brake on the discovery of novel therapies for CF infection. Here, we present a protocol to perform AST of CF pathogens grown as mature, in vivo-like biofilms in an ex vivo CF lung model comprised of pig bronchiolar tissue and synthetic CF sputum (ex vivo pig lung, EVPL).
Item Type: | Journal Article | ||||||||||||
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Subjects: | R Medicine > RC Internal medicine R Medicine > RM Therapeutics. Pharmacology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
Library of Congress Subject Headings (LCSH): | Antibiotics, Drugs -- Effectiveness, Pseudomonas aeruginosa, Staphylococcus aureus, Cystic fibrosis, Lungs -- Diseases | ||||||||||||
Journal or Publication Title: | Journal of Visualized Experiments | ||||||||||||
Publisher: | JoVE | ||||||||||||
ISSN: | 1940-087X | ||||||||||||
Official Date: | 22 January 2021 | ||||||||||||
Dates: |
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Number: | 167 | ||||||||||||
Article Number: | e62187 | ||||||||||||
DOI: | 10.3791/62187 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Copyright Holders: | Copyright © 2021 JoVE | ||||||||||||
Date of first compliant deposit: | 26 January 2021 | ||||||||||||
Date of first compliant Open Access: | 26 January 2021 | ||||||||||||
RIOXX Funder/Project Grant: |
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