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Influence of DIBMA polymer length on lipid nanodisc formation and membrane protein extraction
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Ball, Lauren E., Riley, Liam J., Hadasha, Waled, Pfukwa, Rueben, Smith, Corinne J., Dafforn, Timothy R. and Klumperman, Bert (2021) Influence of DIBMA polymer length on lipid nanodisc formation and membrane protein extraction. Biomacromolecules, 22 (2). pp. 763-772. doi:10.1021/acs.biomac.0c01538 ISSN 1525-7797.
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WRAP-influence-DIBMA-polymer-length-lipid-nanodisc-formation-membrane-protein-extraction-Smith-2020.pdf - Accepted Version - Requires a PDF viewer. Download (3181Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.biomac.0c01538
Abstract
Polymer-based lipid nanoparticles like styrene-maleic acid lipid particles have revolutionized the study of membrane proteins. More recently, alternative polymers such as poly(diisobutylene-alt-maleic acid) (DIBMA) have been used in this field. DIBMA is commonly synthesized via conventional radical copolymerization. In order to study the influence of its chain length on lipid nanodisc formation and membrane protein extraction, we synthesized DIBMA with molar masses varying from 1.2–12 kDa via RAFT-mediated polymerization. For molar masses in the range of 3–7 kDa, the rate of lipid nanodisc formation was the highest and similar to those of poly(styrene-co-maleic acid) (SMA) and commercially available DIBMA. ZipA solubilization efficiency was significantly higher than for commercially available DIBMA and similar to SMA (circa 75%). Furthermore, RAFT-made DIBMA with a molar mass of 1.2–3.9 kDa showed a much cleaner separation on SDS–PAGE, without the smearing that is typically seen for SMA and commercially available DIBMA.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QP Physiology R Medicine > R Medicine (General) |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Polymers in medicine, Nanocomposites (Materials), Membrane proteins -- Research, Diisobutylene, Lipids -- Biotechnology, Lipids -- Research, Nanoparticles | |||||||||
Journal or Publication Title: | Biomacromolecules | |||||||||
Publisher: | American Chemical Society | |||||||||
ISSN: | 1525-7797 | |||||||||
Official Date: | 8 February 2021 | |||||||||
Dates: |
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Volume: | 22 | |||||||||
Number: | 2 | |||||||||
Page Range: | pp. 763-772 | |||||||||
DOI: | 10.1021/acs.biomac.0c01538 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Reuse Statement (publisher, data, author rights): | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.biomac.0c01538 | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||
Date of first compliant deposit: | 5 February 2021 | |||||||||
Date of first compliant Open Access: | 29 December 2021 | |||||||||
RIOXX Funder/Project Grant: |
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