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Data for Alternative architecture of the E. coli chemosensory array
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Cassidy, Keith, Burt, Alister, Stansfeld, Phillip J. and Gutsche, Irina (2021) Data for Alternative architecture of the E. coli chemosensory array. [Dataset]
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Official URL: http://doi.org/10.5281/zenodo.4302473
Abstract
Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here we present cryo-electron tomography visualisations of native-state chemosensory arrays in E. coli minicells. Strikingly, these arrays exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in E. coli . Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies.
Item Type: | Dataset | |||||||||
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Subjects: | Q Science > QH Natural history Q Science > QR Microbiology R Medicine > RC Internal medicine T Technology > TK Electrical engineering. Electronics Nuclear engineering |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Type of Data: | Experimental data | |||||||||
Library of Congress Subject Headings (LCSH): | Escherichia coli, Chemical ecology, Chemotaxis, Cryoelectronics, Tomography, Molecules -- Models | |||||||||
Publisher: | University of Warwick, School of Life Sciences | |||||||||
Official Date: | 10 March 2021 | |||||||||
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Status: | Not Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Media of Output (format): | .pdb | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Copyright Holders: | University of Warwick, University of Oxford | |||||||||
Description: | Data record consists of data in pdb format. We present molecular models for extended patches of the E. coli chemosensory array. The models display either the canonical p6-symmetric architecture or an alternative p2-symmetric architecture recently observed in E. coli minicells. For more information, please see the associated BioRxiv preprint. Residue coverage: Tsr chemoreceptor (residues 340-440); CheA kinase (residues 264-646), CheW adaptor protein (residues 14-158). PDB naming convention: Tsr chemoreceptors (chain T), CheA kinases (chain A), CheW adaptor proteins (chain W). Individual molecules may be obtained using the 'segname' field in each pdb. |
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