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Uncovering the role of Serendipita indica effectors in enhancing host disease resistance
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Osborne, Rory (2019) Uncovering the role of Serendipita indica effectors in enhancing host disease resistance. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3493361~S15
Abstract
Plant species encounter an innumerable number of microbes in both the soil and the air. Whilst most of these interactions are neutral, some organisms have evolved to infect and proliferate in plant tissues. These microbes are confronted by a complex plant immune system which limits their infective capabilities. In response, highly evolved microbes secrete small proteins known as effectors into their host to promote virulence. Whilst this is true for all plant colonising organisms, not all cause disease. One such example is the root colonising endophytic fungus Serendipita indica which is capable of infecting an enormous range of plant species and in doing so enhances yield and host resistance to disease.
In this thesis, we explored the underlying mechanism behind induced systemic resistance associated with S. indica colonization by studying the effectors it secretes. We identified 150 putative effectors released by the symbiont and applied three complimentary screens to assess their ability to activate beneficial defence pathways. We first identified the plant proteins targeted by these 150 effector candidates in a yeast-2-hybrid screen against 12K Arabidopsis genes, which revealed over 250 binary interactions between the host and the fungus. Secondly, we evaluated how these proteins modulate the activity of markers associated with jasmonic acid and salicylic acid defence signalling in Arabidopsis protoplasts using a high-throughput screening platform. This analysis suggested nearly 100 of these putative effectors are able to affect hormone signalling at the transcriptional level. Finally, we overexpressed the strongest inducers and suppressors of these defence markers in Arabidopsis and phenotyped these plants for improved growth and disease resistance. Here, we present the outcome of this global screen together with biochemical and cell biology analyses, which suggest the ethylene branch of hormone and defence signalling is a major contributor to systemic resistance induced by the fungus.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Plants -- Disease and pest resistance -- Molecular aspects, Endophytic fungi, Plant-microbe relationships -- Molecular aspects, Pathogenic bacteria | ||||
Official Date: | September 2019 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Schäfer, Patrick | ||||
Format of File: | |||||
Extent: | xiv, 190 leaves : illustrations (some colour), colour map | ||||
Language: | eng |
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