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Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides
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Casillo, Angela, Fabozzi, Antonio, Russo Krauss, Irene, Parrilli, Ermenegilda, Biggs, Caroline I., Gibson, Matthew I., Lanzetta, Rosa, Appavou, Marie-Sousai, Radulescu, Aurel, Tutino, Maria L., Paduano, Luigi and Corsaro, Maria M. (2021) Physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides. Biomacromolecules, 22 (4). pp. 1445-1457. doi:10.1021/acs.biomac.0c01659 ISSN 1525-7797.
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Official URL: http://dx.doi.org/10.1021/acs.biomac.0c01659
Abstract
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QC Physics Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Microbial exopolysaccharides, Microbial exopolysaccharides -- Synthesis , Small-angle scattering , Electron microscopy , Ice crystals | ||||||||
Journal or Publication Title: | Biomacromolecules | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 1525-7797 | ||||||||
Official Date: | 12 April 2021 | ||||||||
Dates: |
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Volume: | 22 | ||||||||
Number: | 4 | ||||||||
Page Range: | pp. 1445-1457 | ||||||||
DOI: | 10.1021/acs.biomac.0c01659 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 29 March 2021 | ||||||||
Date of first compliant Open Access: | 1 April 2021 |
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