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Contrasting responses of rhizosphere bacterial, fungal, protist, and nematode communities to nitrogen fertilization and crop genotype in field grown oilseed rape (Brassica napus)
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Picot, Emma, Hale, Chris C., Hilton, Sally, Teakle, Graham R., Schäfer, Hendrik, Huang, Yong-Ju, Perryman, Sarah, West, Jon S. and Bending, G. D. (2021) Contrasting responses of rhizosphere bacterial, fungal, protist, and nematode communities to nitrogen fertilization and crop genotype in field grown oilseed rape (Brassica napus). Frontiers in Sustainable Food Systems, 5 . 613269. doi:10.3389/fsufs.2021.613269 ISSN 2571-581X.
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WRAP-contrasting-responses-rhizosphere-bacterial-fungal-protist-nematode-communities-nitrogen-fertilization-crop-genotype-field-grown-oilseed-rape-Bending-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2323Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fsufs.2021.613269
Abstract
The rhizosphere microbiome is considered to play a key role in determining crop health. However, current understanding of the factors which shape assembly and composition of the microbiome is heavily biased toward bacterial communities, and the relevance for other microbial groups is unclear. Furthermore, community assembly is determined by a variety of factors, including host genotype, environment and agricultural management practices, and their relative importance and interactions remain to be elucidated. We investigated the impact of nitrogen fertilization on rhizosphere bacterial, fungal, nematode and protist communities of 10 contrasting oilseed rape genotypes in a field experiment. We found significant differences in the composition of bacteria, fungi, protist and nematode communities between the rhizosphere and bulk soil. Nitrogen application had a significant but weak effect on fungal, bacterial, and protist community composition, and this was associated with increased relative abundance of a complex of fungal pathogens in the rhizosphere and soil, including Mycosphaerella sp. and Leptosphaeria sp. Network analysis showed that nitrogen application had different effects on microbial community connectivity in the soil and rhizosphere. Crop genotype significantly affected fungal community composition, with evidence for a degree of genotype specificity for a number of pathogens, including L. maculans, Alternaria sp., Pyrenopeziza brassicae, Olpidium brassicae, and L. biglobosa, and also potentially beneficial Heliotales root endophytes. Crop genotype had no significant effect on assembly of bacteria, protist or nematode communities. There was no relationship between genetic distance of crop genotypes and the extent of dissimilarity of rhizosphere microbial communities. Field disease assessment confirmed infection of crops by Leptosphaeria sp., P. brassicae, and Alternaria sp., indicating that rhizosphere microbiome sequencing was an effective indicator of plant health. We conclude that under field conditions soil and rhizosphere nutrient stoichiometry and crop genotype are key factors determining crop health by influencing the infection of roots by pathogenic and mutualistic fungal communities, and the connectivity and stability of rhizosphere microbiome interaction networks.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QK Botany S Agriculture > S Agriculture (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Rhizosphere , Rhizobacteria , Nitrogen fertilizers , Nitrogen in agriculture , Rape (Plant) -- Diseases and pests , Pathogenic fungi | ||||||||||||
| Journal or Publication Title: | Frontiers in Sustainable Food Systems | ||||||||||||
| Publisher: | Frontiers Media | ||||||||||||
| ISSN: | 2571-581X | ||||||||||||
| Official Date: | 8 April 2021 | ||||||||||||
| Dates: |
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| Volume: | 5 | ||||||||||||
| Article Number: | 613269 | ||||||||||||
| DOI: | 10.3389/fsufs.2021.613269 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
| Date of first compliant deposit: | 19 April 2021 | ||||||||||||
| Date of first compliant Open Access: | 21 April 2021 | ||||||||||||
| RIOXX Funder/Project Grant: |
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