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Characterization of highly proliferative decidual precursor cells during the window of implantation in human endometrium
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Diniz-da-Costa, Maria, Kong, Chow-Seng, Fishwick, Katherine, Rawlings, Thomas, Brighton, Paul (Paul J.), Hawkes, Amelia, Odendaal, Joshua, Quenby, Siobhan, Ott, Sascha, Lucas, Emma S., Vrljicak, Pavle and Brosens, Jan J. (2021) Characterization of highly proliferative decidual precursor cells during the window of implantation in human endometrium. Stem Cells, 39 (8). pp. 1067-1080. doi:10.1002/stem.3367 ISSN 1066-5099.
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WRAP-characterization-highly-proliferative-decidual-precursor-cells-window-implantation-human-endometrium-Brighton-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3442Kb) | Preview |
Official URL: https://doi.org/10.1002/stem.3367
Abstract
Pregnancy depends on the wholesale transformation of the endometrium, a process driven by differentiation of endometrial stromal cells (EnSC) into specialist decidual cells. Upon embryo implantation, decidual cells impart the tissue plasticity needed to accommodate a rapidly growing conceptus and invading placenta, although the underlying mechanisms are unclear. Here we characterize a discrete population of highly proliferative mesenchymal cells (hPMC) in midluteal human endometrium, coinciding with the window of embryo implantation. Single-cell transcriptomics demonstrated that hPMC express genes involved in chemotaxis and vascular transmigration. Although distinct from resident EnSC, hPMC also express genes encoding pivotal decidual transcription factors and markers, most prominently prolactin. We further show that hPMC are enriched around spiral arterioles, scattered throughout the stroma, and occasionally present in glandular and luminal epithelium. The abundance of hPMC correlated with the in vitro colony-forming unit activity of midluteal endometrium and, conversely, clonogenic cells in culture express a gene signature partially conserved in hPMC. Cross-referencing of single-cell RNA-sequencing data sets indicated that hPMC differentiate into a recently discovered decidual subpopulation in early pregnancy. Finally, we demonstrate that recurrent pregnancy loss is associated with hPMC depletion. Collectively, our findings characterize midluteal hPMC as novel decidual precursors that are likely derived from circulating bone marrow-derived mesenchymal stem/stromal cells and integral to decidual plasticity in pregnancy.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QM Human anatomy Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RG Gynecology and obstetrics |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||
| Library of Congress Subject Headings (LCSH): | B cells, Mesenchymal stem cells , Infertility, Female -- Treatment, Human embryo -- Transplantation, Endometrium , Miscarriage, Pregnancy , Pregnancy -- Complications | |||||||||
| Journal or Publication Title: | Stem Cells | |||||||||
| Publisher: | Oxford University Press | |||||||||
| ISSN: | 1066-5099 | |||||||||
| Official Date: | August 2021 | |||||||||
| Dates: |
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| Volume: | 39 | |||||||||
| Number: | 8 | |||||||||
| Page Range: | pp. 1067-1080 | |||||||||
| DOI: | 10.1002/stem.3367 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 25 May 2021 | |||||||||
| Date of first compliant Open Access: | 26 May 2021 | |||||||||
| RIOXX Funder/Project Grant: |
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