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Stable transformant of Phalaenopsis amabilis somatic embryo carrying 35S::AtRKD4 develops into normal phenotype of transgenic plant
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Perdana, Naufal Ghozi Aditya, Mose, Windi, Lawrie, Muhammad Dylan, Gutierrez-Marcos, José and Semiarti, Endang (2021) Stable transformant of Phalaenopsis amabilis somatic embryo carrying 35S::AtRKD4 develops into normal phenotype of transgenic plant. Journal of Tropical Biodiversity and Biotechnology, 6 (2). 59210. doi:10.22146/jtbb.59210 ISSN 2540-9573.
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WRAP-Stable-transformant-of-Phalaenopsis-amabilis-somatic-embryo-carrying-35S-AtRKD4-develops-into-normal-phenotype-of-transgenic-plant-Gutierrez-Marcos-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons: Attribution-Share Alike 4.0. Download (575Kb) | Preview |
Official URL: https://doi.org/10.22146/jtbb.59210
Abstract
Phalaenopsis amabilis (L.) Blume orchid is an Indonesian national flower. The number of these orchids in their natural habitat is very limited, therefore plant propagation efforts are needed. One of the promising methods is plant propagation by inserting embryo gene AtRKD4 from a model plant Arabidopsis thaliana into the orchid genome to produce many somatic embryos. From previous research, we have obtained 28 plant P. amabilis transformants carrying the AtRKD4 gene, however, it was unknown whether these plants have normal phenotypes and growth similar to their parents. Therefore, descriptions on growth and morphology are needed. This research aimed to evaluate the phenotype of P. amabilis carrying 35S::AtRKD4 the transformants grown in greenhouse. To achieve it, AtRKD4 gene integration stability on transformants genome was analyzed. Morphology and cross-section anatomy structure on transformant and non-transformant plantswere described. The stability of AtRKD4 gene integration in the plant genome was confirmed by amplification of the AtRKD4 gene from genomic DNA with Polymerase Chain Reaction (PCR) using a specific primer for AtRKD4 and ACTIN genes as the internal control. The quantitative data from morphology and anatomy measurements were analyzed statistically using ANOVA. The results showed that AtRKD4 was stably integrated into the genome of P. amabilis transformants and all transformant plants showed similar morphology and anatomy characteristics as non-transformant plants. The AtRKD4 embryo gene was stably integrated into the orchid genome and the transformant plants grow normally without significant changes in phenotype.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Orchids, Arabidopsis -- Genetic engineering, Orchids -- Biotechnology, Phalaenopsis -- Growth, Orchid culture -- Research, Somatic embryogenesis, Plant physiology -- Research | |||||||||
Journal or Publication Title: | Journal of Tropical Biodiversity and Biotechnology | |||||||||
Publisher: | UGM | |||||||||
ISSN: | 2540-9573 | |||||||||
Official Date: | 20 May 2021 | |||||||||
Dates: |
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Volume: | 6 | |||||||||
Number: | 2 | |||||||||
Number of Pages: | 11 | |||||||||
Article Number: | 59210 | |||||||||
DOI: | 10.22146/jtbb.59210 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 21 February 2023 | |||||||||
Date of first compliant Open Access: | 22 February 2023 | |||||||||
RIOXX Funder/Project Grant: |
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