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The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish
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Vong, Yin Ho, Sivashanmugam, Lavanya, Leech, Rebecca, Zaucker, Andreas, Jones, Alexandra M. and Sampath, Karuna (2021) The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish. PLoS Genetics, 17 (7). e1009667. doi:10.1371/journal.pgen.1009667 ISSN 1553-7390.
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WRAP-RNA-binding-protein-Igf2bp3-critical-embryonic-germline-development-zebrafish-Jones-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (4Mb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pgen.1009667
Abstract
The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during early embryonic development and germline development. Loss of Igf2bp3 leads to an expanded yolk syncytial layer (YSL) in early embryos, reduced germline RNA expression, and mis-regulated germline development. We show that loss of maternal Igf2bp3 function results in translational de-regulation of a Nodal reporter during the mid-blastula transition. Furthermore, maternal igf2bp3 mutants exhibit reduced expression of germplasm transcripts, defects in chemokine guidance, abnormal PGC behavior and germ cell death. Consistently, adult igf2bp3 mutants show a strong male bias. Our findings suggest that Igf2bp3 is essential for normal embryonic and germline development, and acts as a key regulator of sexual development.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Zebra danio, Zebra danio -- Behavior -- Genetics, Zebra danio -- Molecular genetics, Germ cells, Gametes -- Evolution, RNA-protein interactions, Protein binding | |||||||||||||||
| Journal or Publication Title: | PLoS Genetics | |||||||||||||||
| Publisher: | Public Library of Science | |||||||||||||||
| ISSN: | 1553-7390 | |||||||||||||||
| Official Date: | 2 July 2021 | |||||||||||||||
| Dates: |
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| Volume: | 17 | |||||||||||||||
| Number: | 7 | |||||||||||||||
| Article Number: | e1009667 | |||||||||||||||
| DOI: | 10.1371/journal.pgen.1009667 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 21 July 2021 | |||||||||||||||
| Date of first compliant Open Access: | 21 July 2021 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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