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Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration
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Larocque, Gabrielle, Moore, Daniel J., Sittewelle, Méghane, Kuey, Cansu, Hetmanski, Joseph H. R., La-Borde, Penelope J., Wilson, Beverley J., Clarke, Nicholas I., Caswell, Patrick T. and Royle, Stephen J. (2021) Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration. The Journal of Cell Biology, 220 (10). e202009028. doi:10.1083/jcb.202009028 ISSN 0021-9525.
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Official URL: https://doi.org/10.1083/jcb.202009028
Abstract
Membrane traffic is an important regulator of cell migration through the endocytosis and recycling of cell surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs) are transport vesicles that are involved in multiple membrane trafficking steps, including the recycling pathway. The only known marker for INVs is tumor protein D54 (TPD54/TPD52L2), a member of the TPD52-like protein family. Overexpression of TPD52-like family proteins in cancer has been linked to poor prognosis and an aggressive metastatic phenotype, which suggests cell migration may be altered under these conditions. Here, we show that TPD54 directly binds membrane and associates with INVs via a conserved positively charged motif in its C terminus. We describe how other TPD52-like proteins are also associated with INVs, and we document the Rab GTPase complement of all INVs. Depletion of TPD52-like proteins inhibits cell migration and invasion, while their overexpression boosts motility. We show that inhibition of migration is likely due to altered recycling of α5β1 integrins in INVs.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Cancer, Cell migration, Biological transport -- Regulation, Membranes (Biology), Cells -- Motility | |||||||||||||||
Journal or Publication Title: | The Journal of Cell Biology | |||||||||||||||
Publisher: | Rockefeller University Press | |||||||||||||||
ISSN: | 0021-9525 | |||||||||||||||
Official Date: | 21 July 2021 | |||||||||||||||
Dates: |
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Volume: | 220 | |||||||||||||||
Number: | 10 | |||||||||||||||
Article Number: | e202009028 | |||||||||||||||
DOI: | 10.1083/jcb.202009028 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 9 August 2022 | |||||||||||||||
Date of first compliant Open Access: | 16 August 2022 | |||||||||||||||
RIOXX Funder/Project Grant: |
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