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Gas chromatography-mass spectrometry analysis of compounds emitted by pepper yellow leaf curl virus-infected chili plants : a preliminary study
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Agustika, Dyah, Mercuriani, Ixora S., Ariyanti, Nur Aeni, Purnomo, Chandra W., Triyana, Kuwat, Iliescu, Daciana and Leeson, Mark S. (2021) Gas chromatography-mass spectrometry analysis of compounds emitted by pepper yellow leaf curl virus-infected chili plants : a preliminary study. Separations, 8 (9). 136. doi:10.3390/separations8090136 ISSN 2297-8739.
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WRAP-gas-chromatography-mass-spectrometry-analysis-compounds-emitted-pepper-yellow-leaf-curl-virus-infected-chili-plants-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3452Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/separations8090136
Abstract
Pepper yellow leaf curl virus (PYLCV) is a threat to chili plants and can significantly reduce yields. This study aimed as a pilot project to detect PYLCV by analyzing compounds emitted by chili plants using gas chromatography-mass spectrometry (GC-MS). The samples investigated in this research were PYLCV-infected and PYLCV-undetected chili plants taken from commercial chili fields. The infection status was validated by using a polymerase chain reaction (PCR) test. A headspace technique was used to extract the volatile organic compounds emitted by plants. The analysis of GC-MS results began with pre-processing, analyzing sample compound variability with a boxplot analysis, and sample classification by using a multivariate technique. Unsupervised multivariate technique principal component analysis (PCA) was performed to discover whether GC-MS could identify PYLCV-infected or not. The results showed that PYLCV-infected and PYLCV-undetected chili plants could be differentiated, with a total percent variance of the first three principal components reaching 91.32%, and successfully discriminated between PYLCV-infected and PYLCV-undetected chili plants. However, more comprehensive studies are needed to find the potential biomarkers of the infected plants.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QK Botany S Agriculture > SB Plant culture T Technology > TD Environmental technology. Sanitary engineering |
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| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||
| Library of Congress Subject Headings (LCSH): | Peppers -- Diseases and pests , Plant diseases , Phytopathogenic microorganisms -- Control , Leaves -- Diseases and pests , Volatile organic compounds, Volatile organic compounds -- Control, Gas chromatography , Mass spectrometry | ||||||
| Journal or Publication Title: | Separations | ||||||
| Publisher: | MDPI | ||||||
| ISSN: | 2297-8739 | ||||||
| Official Date: | 27 August 2021 | ||||||
| Dates: |
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| Volume: | 8 | ||||||
| Number: | 9 | ||||||
| Article Number: | 136 | ||||||
| DOI: | 10.3390/separations8090136 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 1 September 2021 | ||||||
| Date of first compliant Open Access: | 2 September 2021 | ||||||
| RIOXX Funder/Project Grant: |
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