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Enzymatic hydrolysis of bacterial cellulose in the presence of a non‐catalytic cerato‐platanin protein
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Rovera, Cesare, Luti, Simone, Pazzagli, Luigia, Heeley, Ellen L., Wan, Chaoying, Romano, Diego and Farris, Stefano (2022) Enzymatic hydrolysis of bacterial cellulose in the presence of a non‐catalytic cerato‐platanin protein. Journal of Applied Polymer Science, 139 (14). 51886. doi:10.1002/app.51886 ISSN 0021-8995.
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Official URL: https://doi.org/10.1002/app.51886
Abstract
In this work, the effect of an expansin‐like cerato‐platanin (CP) protein as a pre‐treatment for the enzymatic hydrolysis of bacterial cellulose (BC) is investigated. To this scope, cellulases from Trichoderma reesei are used as hydrolyzing agent using different enzyme/BC formulations. Turbidity experiments reveal that for the higher enzyme concentrations (formulations 0.5:1 and 1:1) the enzymatic hydrolysis of BC show similar hydrolysis kinetics and is not dependent of the CP. However, at higher BC concentrations (formulations 0.25:1 and 0.33:1), the hydrolysis of BC is hindered by the non‐catalytic protein, as confirmed by the lower content of cellobiose and glucose in the presence of CP. Light scattering experiments show that the addition of CP led to an increase of the BC particle size from (445–630 nm) to (890–1.26 μm) for the formulation 1:1, which is also corroborated by atomic force microscopy and transmission electron microscopy analyses. These results suggest that CP did not positively affect the hydrolysis of BC, in contrast to what was previously observed for plant‐derived cellulose. This work for the first time investigates the anomalous behavior of cerato platanin family members with regard to its loosening activity on the structure of bacterial cellulose.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology T Technology > TA Engineering (General). Civil engineering (General) T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Library of Congress Subject Headings (LCSH): | Biopolymers, Hydrolysis, Ceratocystis , Bacteria -- Ecology, Cellulose -- Chemistry, Nanostructured materials | ||||||||
Journal or Publication Title: | Journal of Applied Polymer Science | ||||||||
Publisher: | John Wiley & Sons, Inc. | ||||||||
ISSN: | 0021-8995 | ||||||||
Official Date: | 10 April 2022 | ||||||||
Dates: |
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Volume: | 139 | ||||||||
Number: | 14 | ||||||||
Article Number: | 51886 | ||||||||
DOI: | 10.1002/app.51886 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 10 November 2021 | ||||||||
Date of first compliant Open Access: | 11 November 2021 | ||||||||
RIOXX Funder/Project Grant: |
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