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Lateral flow glyco‐assays for the rapid and low‐cost detection of lectins–polymeric linkers and particle engineering are essential for selectivity and performance
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Baker, Alexander N., Muguruza, Asier R., Richards, Sarah‐Jane, Georgiou, Panagiotis G., Goetz, Stephen, Walker, Marc, Dedola, Simone, Field, Robert A. and Gibson, Matthew I. (2022) Lateral flow glyco‐assays for the rapid and low‐cost detection of lectins–polymeric linkers and particle engineering are essential for selectivity and performance. Advanced Healthcare Materials, 11 (4). 2101784. doi:10.1002/adhm.202101784 ISSN 2192-2659.
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WRAP-Lateral-flow-glyco-assays-low-cost-lectins–polymeric-particle-engineering-performance-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3878Kb) | Preview |
Official URL: https://doi.org/10.1002/adhm.202101784
Abstract
Lateral flow immuno‐assays, such as the home pregnancy test, are rapid point‐of‐care diagnostics that use antibody‐coated nanoparticles to bind antigens/analytes (e.g., viruses, toxins or hormones). Ease of use, no need for centralized infrastructure and low‐cost, makes these devices appealing for rapid disease identification, especially in low‐resource environments. Here glycosylated polymer‐coated nanoparticles are demonstrated for the sensitive, label‐free detection of lectins in lateral flow and flow‐through. The systems introduced here use glycans, not antibodies, to provide recognition: a “lateral flow glyco‐assay,” providing unique biosensing opportunities. Glycans are installed onto polymer termini and immobilized onto gold nanoparticles, providing colloidal stability but crucially also introducing assay tunability and selectivity. Using soybean agglutinin and Ricinus communis agglutinin I (RCA120) as model analytes, the impact of polymer chain length and nanoparticle core size are evaluated, with chain length found to have a significant effect on signal generation—highlighting the need to control the macromolecular architecture to tune response. With optimized systems, lectins are detectable at subnanomolar concentrations, comparable to antibody‐based systems. Complete lateral flow devices are also assembled to show how these devices can be deployed in the “real world.” This work shows that glycan‐binding can be a valuable tool in rapid diagnostics.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Rapid methods (Microbiology) , Point-of-care testing , Gold -- Immunology, Nanoparticles -- Therapeutic use, Lectins | |||||||||||||||||||||
| Journal or Publication Title: | Advanced Healthcare Materials | |||||||||||||||||||||
| Publisher: | Wiley | |||||||||||||||||||||
| ISSN: | 2192-2659 | |||||||||||||||||||||
| Official Date: | 16 February 2022 | |||||||||||||||||||||
| Dates: |
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| Volume: | 11 | |||||||||||||||||||||
| Number: | 4 | |||||||||||||||||||||
| Article Number: | 2101784 | |||||||||||||||||||||
| DOI: | 10.1002/adhm.202101784 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 23 November 2021 | |||||||||||||||||||||
| Date of first compliant Open Access: | 24 November 2021 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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