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Dipolar order parameters in large systems with fast spinning
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Franks, W. Trent, Tatman, Ben P., Trenouth, Jonah and Lewandowski, Józef R. (2021) Dipolar order parameters in large systems with fast spinning. Frontiers in Molecular Biosciences, 8 . 791026. doi:10.3389/fmolb.2021.791026 ISSN 2296-889X.
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WRAP-Dipolar-order-parameters-large-systems-fast-spinning-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1907Kb) | Preview |
Official URL: https://doi.org/10.3389/fmolb.2021.791026
Abstract
Order parameters are a useful tool for quantifying amplitudes of molecular motions. Here we measure dipolar order parameters by recoupling heteronuclear dipole-dipole couplings under fast spinning. We apply symmetry based recoupling methods to samples spinning under magic angle at 60 kHz by employing a variable flip angle compound inversion pulse. We validate the methods by measuring site-specific 15N-1H order parameters of a microcrystalline protein over a small temperature range and the same protein in a large, precipitated complex with antibody. The measurements of the order parameters in the complex are consistent with the observed protein undergoing overall motion within the assembly.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QC Physics Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics |
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| Library of Congress Subject Headings (LCSH): | Protons -- Capture, Statistical mechanics, Nuclear magnetic resonance spectroscopy, Solid state chemistry, Membrane proteins -- Spectroscopic imaging | |||||||||||||||
| Journal or Publication Title: | Frontiers in Molecular Biosciences | |||||||||||||||
| Publisher: | Frontiers Media | |||||||||||||||
| ISSN: | 2296-889X | |||||||||||||||
| Official Date: | 9 December 2021 | |||||||||||||||
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| Volume: | 8 | |||||||||||||||
| Article Number: | 791026 | |||||||||||||||
| DOI: | 10.3389/fmolb.2021.791026 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Copyright Holders: | W. Trent Franks, Ben P. Tatman, Jonah Trenouth, Józef R. Lewandowski | |||||||||||||||
| Date of first compliant deposit: | 9 December 2021 | |||||||||||||||
| Date of first compliant Open Access: | 13 December 2021 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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