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Data for Polymer-mediated cryopreservation of bacteriophages
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Marton, Huba L., Styles, Kathryn, Kilbride, Peter, Sagona, Antonia P. and Gibson, Matthew I. (2021) Data for Polymer-mediated cryopreservation of bacteriophages. [Dataset]
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Official URL: http://wrap.warwick.ac.uk/160983/
Abstract
Bacteriophages (phages, bacteria-specific viruses) have biotechnological and therapeutic potential. To apply phages as pure or heterogeneous mixtures, it is essential to have a robust mechanism for transport and storage, with different phages having very different stability profiles across storage conditions. For many biologics, cryopreservation is employed for long-term storage and cryoprotectants are essential to mitigate cold-induced damage. Here, we report that poly(ethylene glycol) can be used to protect phages from cold damage, functioning at just 10 mg·mL–1 (∼1 wt %) and outperforms glycerol in many cases, which is a currently used cryoprotectant. Protection is afforded at both −20 and −80 °C, the two most common temperatures for frozen storage in laboratory settings. Crucially, the concentration of the polymer required leads to frozen solutions at −20 °C, unlike 50% glycerol (which results in liquid solutions). Post-thaw recoveries close to 100% plaque-forming units were achieved even after 2 weeks of storage with this method and kill assays against their bacterial host confirmed the lytic function of the phages. Initial experiments with other hydrophilic polymers also showed cryoprotection, but at this stage, the exact mechanism of this protection cannot be concluded but does show that water-soluble polymers offer an alternative tool for phage storage. Ice recrystallization inhibiting polymers (poly(vinyl alcohol)) were found to provide no additional protection, in contrast to their ability to protect proteins and microorganisms which are damaged by recrystallization. PEG’s low cost, solubility, well-established low toxicity/immunogenicity, and that it is fit for human consumption at the concentrations used make it ideal to help translate new approaches for phage therapy.
Item Type: | Dataset | ||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Library of Congress Subject Headings (LCSH): | Bacteriophages, Microorganisms -- Cryopreservation, Polyethylene glycol, Water-soluble polymers | ||||||||||||||||||
Publisher: | University of Warwick, Department of Chemistry | ||||||||||||||||||
Official Date: | 9 December 2021 | ||||||||||||||||||
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Status: | Not Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Media of Output (format): | .xlsx | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Copyright Holders: | University of Warwick | ||||||||||||||||||
Description: | Tabulated data underpinning each figure is included. |
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Date of first compliant deposit: | 9 December 2021 | ||||||||||||||||||
Date of first compliant Open Access: | 9 December 2021 | ||||||||||||||||||
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